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Small Cationic Protein From a Marine Turtle Has �- Defensin-Like Fold and Antibacterial and Antiviral Activity

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Title Small Cationic Protein From a Marine Turtle Has �-
Defensin-Like Fold and Antibacterial and Antiviral Activity
 
Creator Chattopadhyay, Suranjana
Sinha, Nirmal Kumar
Banerjee, Shuvojit
Roy, Debjani
Chattopadhyay, Dhrubajyoti
Roy, Siddhartha
 
Subject Structural Biology & Bioinformatics
 
Description Egg white of marine turtle Caretta
caretta contains a small cationic protein but lacks
lysozyme. The protein was sequenced by a combination
of sequential Edman degradation, carboxypeptidase
digestion, nuclear magnetic resonance (NMR)
and electrospray ionization tandem mass spectrometry.
The protein contains 36 amino acid residues of
which six are half-cysteines. The three-dimensional
structure of the protein was deduced from twodimensional
NMR experiments and was observed to
be similar to vertebrate �-defensins. However, disulfide
connectivity is C1–C6/C2–C5/C3–C4; different
from that of the vertebrate �-defensins. The protein
showed strong antibacterial activity against Escherichia
coli and Salmonella typhimurium. The protein
also showed significant antiviral activity against
an enveloped rhabdovirus, Chandipura virus, which
is an emerging human pathogen. This virus is also
closely related to the vesicular stomatitis virus,
whose growth was also inhibited. This small cationic
protein is part of the innate immunity of this
organism and replaces lysozyme in the egg. It has
the potential to be developed as an antibacterial
and antiviral agent. Proteins 2006;64:524–531.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/485/1/PROTEINS%2DSTRUCTURE_FUNCTION_AND_BIOINFORMATICS%2C_64(_2)%2C524%2D531%2C[52].pdf
Chattopadhyay, Suranjana and Sinha, Nirmal Kumar and Banerjee, Shuvojit and Roy, Debjani and Chattopadhyay, Dhrubajyoti and Roy, Siddhartha (2006) Small Cationic Protein From a Marine Turtle Has �- Defensin-Like Fold and Antibacterial and Antiviral Activity. "Proteins: Structure, Function, and Bioinformatics", 64 (2). pp. 524-531.
 
Relation http://dx.doi.org/10.1002/prot.20963
http://www.eprints.iicb.res.in/485/