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Characterization of Immunoglobulin G and Its Subclass Response to Indian Kala-Azar Infection before and after Chemotherapy

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Title Characterization of Immunoglobulin G and Its Subclass Response to
Indian Kala-Azar Infection before and after Chemotherapy
 
Creator Ravindran, Rajesh Ravindran
Anam, Khairul
Bairagi, Bibhas C
Saha, Bibhuti
Pramanik, Netai
Guha, Subhasis K
Goswami, Rama Prosad
Banerjee, Dwijadas
Ali, Nahid
 
Subject Infectious Diseases and Immunology
 
Description Serologic parameters of kala-azar were evaluated by Western blot analysis. Sera from kala-azar patients
with confirmed diagnoses were screened for immunoglobulin G (IgG) and IgG subclass-specific reactivity
against Leishmania donovani membrane antigen (LAg). Heterogenous LAg-specific IgG reactivity with numerous
proteins with molecular masses ranging from 18 to 190 kDa was observed. Though the individual band
patterns were varied, seven polypeptides of approximately 31, 34, 51, 63, 72, 91, and 120 kDa were immunoreactive
with all the sera tested from kala-azar patients. The band patterns of the immunoblots of sera from
patients after treatment and clinical cure with sodium antimony gluconate revealed a decrease in the frequency
of the bands. Still, recognition of the 63- and 120-kDa bands was 100%, and the 55- and 91-kDa fractions were
recognized in 93% of the sera from cured individuals. Among the IgG subclasses, IgG1 reacted with the greatest
number of polypeptides. The 63-kDa protein was again detected by all of the IgG subclasses of all the sera
tested. Other fractions recognized by the subclasses of more than 70% of the serum samples included those of
47, 51, 55, and 78 kDa. Following treatment, 63- and 51-kDa bands were the most reactive with the IgG
subclasses. LAg-associated cross-reaction with other reference human antisera revealed a mild reactivity of the
63-kDa polypeptide with some of the serum samples from leprosy, malaria, typhoid, tuberculosis, and healthy
controls. Western blot analysis of LAg entrapped in liposomes, strong vaccine candidates against experimental
visceral leishmaniasis, revealed a more restricted band pattern. The 63-kDa fraction revealed by all pre- and
posttreatment sera showed almost negligible levels of cross-reaction with sera from patients with other diseases
or from healthy controls. These observations provide insight into induced immunity during kala-azar infection
for future application.
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/536/1/INFECTION_AND_IMMUNITY%2C_72(_2)%2C_863%2D870[82].pdf
Ravindran, Rajesh Ravindran and Anam, Khairul and Bairagi, Bibhas C and Saha, Bibhuti and Pramanik, Netai and Guha, Subhasis K and Goswami, Rama Prosad and Banerjee, Dwijadas and Ali, Nahid (2004) Characterization of Immunoglobulin G and Its Subclass Response to Indian Kala-Azar Infection before and after Chemotherapy. Infection and Immunity, 72 (2). pp. 863-870.
 
Relation http://dx.doi.org/10.1128/IAI.72.2.863–870.2004
http://www.eprints.iicb.res.in/536/