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Differential Expression of 9-o-aAcetylated Sialoglycoconjugates on Leukemic blasts: a Potential tool for long-term Monitoring of Children with Acute Lymphoblastic Leukemia

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Title Differential Expression of 9-o-aAcetylated Sialoglycoconjugates on Leukemic blasts: a Potential tool for long-term Monitoring of Children with Acute Lymphoblastic Leukemia
 
Creator Pal, Santanu
Ghosh, Shyamasree
Bandyopadhyay, Suman
Mandal, Chhabinath
Bandyopadhyay, Santu
Bhattacharyya, Dilip Kumar
Mandal, Chitra
 
Subject Drug Development/Diagnostics & Biotechnology
Infectious Diseases and Immunology
 
Description Earlier studies have demonstrated overexpression of 9-Oacetylated
sialoglycoconjugates (9-O-AcSGs) on lymphoblasts,
concomitant with high titers of anti-9-O-AcSG antibodies
in childhood acute lymphoblastic leukemia (ALL).
Our aim was to evaluate the correlation between expression
of different 9-O-AcSGs during chemotherapeutic treatment.
Accordingly, expression of 9-O-AcSGs on lymphoblasts of
ALL patients (n � 70) were longitudinally monitored for 6
years (1997–2002), using Achatinin-H, a 9-O-acetylated sialic
acid (9-O-AcSA) binding lectin with preferential affinity for
9-O-AcSGs with terminal 9-O-AcSA�236GalNAc. Western
blot analysis of patients (n � 30) showed that 3 ALL-specific
9-O-AcSGs (90, 120 and 135 kDa) were induced at presentation;
all these bands disappeared after treatment in patients
(n � 22) who had disease-free survival. The 90 kDa band
persisted in 8 patients who subsequently relapsed with reexpression
of the 120 kDa band. FACS analysis revealed that at
presentation (n � 70) 90.1 � 5.0% cells expressed 9-O-AcSGs,
which decreased progressively with chemotherapy, remained
 
Publisher John Wiley & Sons
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/537/1/INTERNATIONAL_JOURNAL_OF_CANCER%2C_111(_2)%2C_270%2D277_[35].pdf
Pal, Santanu and Ghosh, Shyamasree and Bandyopadhyay, Suman and Mandal, Chhabinath and Bandyopadhyay, Santu and Bhattacharyya, Dilip Kumar and Mandal, Chitra (2004) Differential Expression of 9-o-aAcetylated Sialoglycoconjugates on Leukemic blasts: a Potential tool for long-term Monitoring of Children with Acute Lymphoblastic Leukemia. International Journal of Cancer, 111 (2). pp. 270-277. ISSN 0020-7136
 
Relation http://dx.doi.org/10.1002/ijc.20246
http://www.eprints.iicb.res.in/537/