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Studies on the Production and Purification of an Antimicrobial Compound and Taxonomy of the Producer Isolated from the Marine Environment of the Sundarbans

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Title Studies on the Production and Purification of an Antimicrobial Compound and Taxonomy of the Producer Isolated
from the Marine Environment of the Sundarbans
 
Creator saha, M
Ghosh, D (Jr)
Ghosh, D
Garai, D
Jaisankar, P
Sarkar, Kalyan K
Dutta, Pradeep K
Das, S
Jha, T
Mukherjee, J
 
Subject Chemistry
 
Description A microorganism isolated from the Sundarbans
region of the Bay of Bengal, India, showed potent
antimicrobial activity against gram-positive and gramnegative bacteria, molds, yeast and several multiple-drugresistant (MDR) bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). The isolate grew in the presence of 20% (w/v) NaCl, antibiotic production being
maximum with 5% (w/v) NaCl in the production medium.
Natural seawater stimulated antibiotic biosynthesis. The
absence of catabolite repression during the synthesis of the
antimicrobial substance was demonstrated by the utilization
of glucose by this isolate. The 16S rRNA gene of this
aerobic, gram-positive, mycelium- and spore-forming
microorganism was amplified, and molecular phylogenetic
analysis of the DNA sequence showed less than 93%
similarity with its closest relative, indicating differentiation at the genus level. The highly stable, active principle was purified by butyl acetate extraction and silica-gel chromatography and a single compound was found to posses the broad-spectrum activity. Molecular characterization showed that the active compound is a lipid. Bioreactor studies demonstrated that antibiotic production is strongly dependent on the scale of operation and there is a definite relation between the dissolved oxygen concentration, medium pH, glucose utilization, cell differentiation and antibiotic production. Maximum production in 30 h could be obtained by regulation of the medium pH in the alkaline range by a combination of controlled addition of NaOH, regulation of the air supply and changes in the reactor configuration. Considering all of the above evidences and based on comparison with the current literature, a novel antimicrobial appears to have been isolated.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/551/1/APPLIED_MICROBIOLOGY_AND_BIOTECHNOLOGY__66(_5)497%2D505;2005[97].pdf
saha, M and Ghosh, D (Jr) and Ghosh, D and Garai, D and Jaisankar, P and Sarkar, Kalyan K and Dutta, Pradeep K and Das, S and Jha, T and Mukherjee, J (2005) Studies on the Production and Purification of an Antimicrobial Compound and Taxonomy of the Producer Isolated from the Marine Environment of the Sundarbans. Applied Microbiology and Biotechnology, 66 (5). pp. 497-505.
 
Relation http:dx.doi.org/10.1007/s00253-004-1706-3
http://www.eprints.iicb.res.in/551/