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Berberine, a strong polyriboadenylic acid binding plant alkaloid: spectroscopic, viscometric, and thermodynamic study

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Title Berberine, a strong polyriboadenylic acid binding plant alkaloid:
spectroscopic, viscometric, and thermodynamic study
 
Creator Yadav, Ram Chandra
Kumar, G Suresh
Bhadra, Kakali
Giri, Prabal
Sinha, Rangana
Pal, Sumana
Maiti, Motilal
 
Subject Chemistry
 
Description The interaction of berberine with single stranded poly(rA) structure was investigated using a combination of spectrophotometric,
spectrofluorimetric, circular dichroic, viscometric, and thermodynamic studies. The interaction process was characterized
by typical hypochromic and bathochromic effects in the absorption spectrum of berberine, enhancement of fluorescence intensity of
berberine, increase of viscosity, and perturbation of circular dichroic spectrum of single stranded poly(rA). Scatchard plot obtained
from spectrophotometric analysis showed that berberine bound strongly to single stranded poly(rA) in a non-cooperative manner.
In contrast, berberine does not show any significant effect (i) in its absorbance and fluorescence spectra on binding to double
stranded poly(rA), (ii) alter the circular dichroic spectrum of double stranded poly(rA), or (iii) increase of viscosity of double
stranded poly(rA) indicating that it does not bind at all to double stranded poly(rA) structure. Thermodynamic parameters indicated
that the binding of the alkaloid to single stranded poly(rA) is an endothermic process and entropy driven. All these findings,
taken together clearly support that berberine binds strongly to single stranded poly(rA) structure by a mechanism of partial intercalation
leading to its use in gene regulation in eukaryotic cells.
 
Publisher Elsevier
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/560/1/BIOORGANIC_%26_MEDICINAL_CHEMISTRY_13_(1)_165%2D174;2005[105].pdf
Yadav, Ram Chandra and Kumar, G Suresh and Bhadra, Kakali and Giri, Prabal and Sinha, Rangana and Pal, Sumana and Maiti, Motilal (2005) Berberine, a strong polyriboadenylic acid binding plant alkaloid: spectroscopic, viscometric, and thermodynamic study. Bioorganic & Medicinal Chemistry, 13 (1). pp. 165-174.
 
Relation http://dx.doi.org/10.1016/j.bmc.2004.09.045
http://www.eprints.iicb.res.in/560/