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Generation of Novel Plasmids in Escherichia coli S17-1(pSUP106)

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Title Generation of Novel Plasmids in Escherichia coli S17-1(pSUP106)
 
Creator Mahapatra, Nitish R
Ghosh, Sajalendu
Sarkar, Partha K
Banerjee, Pataki C
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description When the highly metal-resistant acidophilic heterotrophic strain, Acidiphilium symbioticum
KM2, was incubated with two Escherichia coli strains, viz. S17-1 (pSUP106) and K12, on a medium that
supported growth of these two divergent species of different habitats, E. coli transconjugants were
isolated that contained novel plasmids and were resistant to Zn2� (48 mM), Cu2� (12 mM), Ni2� (12 mM),
chloramphenicol (50 �g/ml), and tetracycline (25 �g/ml). The transconjugant plasmids did not hybridize
with any of the A. symbioticum KM2 plasmids. After curing of the plasmids, the transconjugants became
sensitive to 12 mM Zn2�, 12 mM Cu2�, and 12 mM Ni2�, but remained chloramphenicol and tetracycline
resistant—the phenotypic markers that were originally present in pSUP106. That a part of pSUP106 was
integrated into the chromosome of the transconjugants was evident from the hybridization of pSUP106
with chromosomal DNA of the cured derivatives of the transconjugants. Further, the transconjugant
plasmids hybridized only with the chromosomal DNA of E. coli S17-1 and not with the chromosomal
DNA of A. symbioticum KM2 or E. coli K12, suggesting their host chromosomal origin. Thus, the present
study describes a unique event of genetic rearrangements in the E. coli strain S17-1 (pSUP106), resulting
in the formation of novel plasmids conferring metal-resistance phenotypes in the cell.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/562/1/CURRENT_MICROBIOLOGY%2C_46(_5)%2C_318%2D323[62].pdf
Mahapatra, Nitish R and Ghosh, Sajalendu and Sarkar, Partha K and Banerjee, Pataki C (2003) Generation of Novel Plasmids in Escherichia coli S17-1(pSUP106). CURRENT MICROBIOLOGY , 46 (5). pp. 318-323.
 
Relation http://dx.doi.org/10.1007/s00284-002-3835-1
http://www.eprints.iicb.res.in/562/