Record Details

Nucleic Acid Sequence Analysis of Basal Core Promoter/Precore/Core Region of Hepatitis B Virus Isolated from Chronic Carriers of the Virus from Kolkata, Eastern India: Low Frequency of Mutation in the Precore Region

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Nucleic Acid Sequence Analysis of Basal Core Promoter/Precore/Core Region of Hepatitis B
Virus Isolated from Chronic Carriers of the Virus
from Kolkata, Eastern India: Low Frequency of
Mutation in the Precore Region
 
Creator Banerjee, Arup
Banerjee, Soma
Chowdhury, Abhijit
Santra, Amal
Chowdhury, Sujit
Roychoudhury, Susanta
Panda, Chinmoy Kumar
Bhattacharya, Sujit Kumar
 
Subject Molecular & Human Genetics
 
Description The aim of the present study was to characterize
the predominant hepatitis B virus (HBV) strains and
their molecular variants present in the HBV isolates of
the different genotypes found among the chronic carriers
of the virus in our community. Methods: Precore/core
and core promoter regions of HBV DNA were amplifi ed
by polymerase chain reaction and then subjected to direct
sequencing. Of the 64 hepatitis B surface antigen
(HBsAg)-positive chronic HBV carriers investigated, 44
were HBeAg negative and 20 were HBeAg positive. Results:
In addition to genotype D, which was the predominant
ge notype, 12 genotype C (18.7%) and 6 genotype
A (9.4%) were also detected. Presence of T at nt 1858 has
often been related to the development of precore stop
mutation at nt 1896, while that of C has been related to
the development of 1762–1764 double mutation. In our
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/573/1/INTERVIROLOGY_48_(_6)_389%2D399_;2005[108].pdf
Banerjee, Arup and Banerjee, Soma and Chowdhury, Abhijit and Santra, Amal and Chowdhury, Sujit and Roychoudhury, Susanta and Panda, Chinmoy Kumar and Bhattacharya, Sujit Kumar (2005) Nucleic Acid Sequence Analysis of Basal Core Promoter/Precore/Core Region of Hepatitis B Virus Isolated from Chronic Carriers of the Virus from Kolkata, Eastern India: Low Frequency of Mutation in the Precore Region. Intervirology, 48 (6). pp. 389-399.
 
Relation http://dx.doi.org/10.1159/000086066
http://www.eprints.iicb.res.in/573/