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Accelerated Publication Role of superoxide dismutase in survival of Leishmania within the macrophage

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Field Value
 
Title Accelerated Publication
Role of superoxide dismutase in survival of Leishmania within
the macrophage
 
Creator Ghosh, Sanjay
Goswami, Srikanta
Adhya, Samit
 
Subject Molecular & Human Genetics
 
Description Intracellular parasitic protozoans of the genus Leishmania depend
for their survival on the elaboration of enzymic and other
mechanisms for evading toxic free-radical damage in¯icted by
their phagocytic macrophage host. One such mechanism may
involve superoxide dismutase (SOD), which detoxi®es reactive
superoxide radicals produced by activated macrophages, but the
role of this enzyme in parasite survival has not yet been
demonstrated. We have cloned a SOD gene from L. tropica and
generated SOD-de®cient parasites by expressing the corresponding antisense RNA from an episomal vector. Such parasites have
enhanced sensitivity to menadione and hydrogen peroxide in
axenic culture, and a markedly reduced survival in mouse
macrophages. These results indicate that SOD is a major
determinant of intracellular survival of Leishmania.
 
Publisher Portland Press
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/593/1/BIOCHEMICAL_JOURNAL%2C369%2C_447%2D452[82].pdf
Ghosh, Sanjay and Goswami, Srikanta and Adhya, Samit (2003) Accelerated Publication Role of superoxide dismutase in survival of Leishmania within the macrophage. Biochemical Journal, 369. pp. 447-452. ISSN 0264-6021
 
Relation http://dx.doi.org/10.1042/BJ20021684
http://www.eprints.iicb.res.in/593/