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Prenatal exposure to high galactose adversely affects initial gonadal pool of germ cells in rats

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Title Prenatal exposure to high galactose adversely affects initial
gonadal pool of germ cells in rats
 
Creator Bandyopadhyay, Santu
Chakrabarti, J
Banerjee, S
Pal, A K
Bhattacharyya, D
Goswami, S K
Chakravarty, B N
Kabir, S N
 
Subject Cell Biology & Physiology
 
Description In rats, prenatal exposure to high concentrations of galactose may contribute to a condition that
is equivalent to the premature ovarian failure (POF) component of human galactosaemia. We investigated if development
of POF under experimental galactosaemia-like conditions was attributed to impaired germ cell migration.
Pregnant rats were fed pellets supplemented with, or without, 35% galactose from day 3 of conception
continuing through parturition. Between days 12±15, embryos from one uterine horn were dissected out. Primordial
germ cells (PGC) were histochemically localized and counted on the basis of binding of Dolichos bi¯orus agglutinin,
a lectin speci®c for terminal N-acetylgalactosamine (GalNAc), to the surface glycoconjugate of the germ cells. The
embryos from the other uterine horn were maintained until parturition. Liver activity of uridine diphosphate galactose
4-epimerase, the enzyme involved at multiple steps in the process of synthesis of GalNAc, was assayed in 1±2
day old female pups.The numbers of PGC at the day-speci®c sites on all days of examination were signi
®cantly lower (P < 0.0003), and liver epimerase activity was signi®cantly (P = 0.000001) reduced in the galactoseexposed
group.Impaired germ cell migration leading to the development of gonads with de®cient
initial pools of germ cells may form the causal link between galactosaemia and POF.
 
Publisher Oxford University Press
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/604/1/HUMAN_REPRODUCTION%2C_18_(2)%2C_276%2D282[83].pdf
Bandyopadhyay, Santu and Chakrabarti, J and Banerjee, S and Pal, A K and Bhattacharyya, D and Goswami, S K and Chakravarty, B N and Kabir, S N (2003) Prenatal exposure to high galactose adversely affects initial gonadal pool of germ cells in rats. Human Reproduction, 18 (2). pp. 276-282.
 
Relation http://dx.doi.org/10.1093/humrep/deg058
http://www.eprints.iicb.res.in/604/