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Structural and functional studies of eukaryotic topoisomerase I with special reference to Leishmania donovani type I DNA topoisomerase

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Title Structural and functional studies of eukaryotic
topoisomerase I with special reference to
Leishmania donovani type I DNA topoisomerase
 
Creator Ganguly, Agneyo
 
Subject Infectious Diseases and Immunology
 
Description Soon after the double-helical structure was proposed for DNA (Watson and Crick, 1953a), the replication of the circular DNA molecule of a prokaryote raised an intriguing question: How did the two sister double-stranded DNA (dsDNA) molecules disentangle (Watson and Crick, 1953b)? This mechanical problem of separating two intertwined chains became a topological one. In such a
molecule, the two single stranded rings are topologically linked, and therefore cannot come apart without at least one transient break in one of the two strands. The order or extent of topological linkage of the two strands is expressed by their linking number (Lk). Lk is defined as the number of times the two single strands revolve around each other. A right handed double helix with n base pairs will make one full turn every 10.5 base pairs under physiological conditions in its
most stable structure. Therefore, its linking number (Lk0) is expected to be n / 10.5; the superscript specifying the most stable state of the molecule
 
Date 2008
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/607/1/AGNEYO_THESIS.pdf
Ganguly, Agneyo (2008) Structural and functional studies of eukaryotic topoisomerase I with special reference to Leishmania donovani type I DNA topoisomerase. PhD thesis, Jadavpur University.
 
Relation http://www.eprints.iicb.res.in/607/