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Differential Decline in Leishmania Membrane Antigen-Specific Immunoglobulin G (IgG), IgM, IgE, and IgG Subclass Antibodies in Indian Kala-Azar Patients after Chemotherapy

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Title Differential Decline in Leishmania Membrane Antigen-Specific
Immunoglobulin G (IgG), IgM, IgE, and IgG Subclass Antibodies
in Indian Kala-Azar Patients after Chemotherapy
 
Creator Anam, Khairul
Afrin, Farhat
Banerjee, Diwadas
Pramanik, Netai
Guha, Subhasis K
Goswami, Rama Prosad
Saha, Shiben K
Ali, Nahid
 
Subject Infectious Diseases and Immunology
 
Description Pathogenesis in kala-azar is associated with depressed cellular immunity and significant elevation of
antileishmanial antibodies. Since these antibodies are present even after cure, analysis of the parasite-specific
isotypes and immunoglobulin G (IgG) subclasses in kala-azar patients may shed new light on the immune
responses during progression and resolution of infection. Using leishmanial membrane antigenic extracts, we
investigated the relative levels of specific IgG, IgM, IgA, IgE, and IgG subclasses in Indian kala-azar patient
sera during disease, drug resistance, and cure. Acute-phase sera showed strong stimulation of IgG, followed by
IgE and IgM and lastly by IgA antibodies. IgG subclass analysis revealed expression of all of the subclasses,
with a predominance of IgG1 during disease. Following sodium stibogluconate (SAG) resistance, the levels of
IgG, IgM, IgE, and IgG4 remained constant, while there was a decrease in the titers of IgG2 and IgG3. In
contrast, a significant (2.2-fold) increase in IgG1 was observed in these individuals. Cure, in both SAGresponsive
and unresponsive patients, correlated with a decline in the levels of IgG, IgM, IgE, and all of the
IgG subclasses. The stimulation of IgG1 and the persistence, most importantly, of IgE and IgG4 following drug
resistance, along with a decline in IgE, IgG4, and IgG1 with cure, demonstrate the potential of these isotypes
as possible markers for monitoring effective treatment in kala-azar.
 
Publisher American Society for Microbiology
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/673/1/INFECTION_AND_IMMUNITY___67__(12)____6663%2D6669;1999[2].pdf
Anam, Khairul and Afrin, Farhat and Banerjee, Diwadas and Pramanik, Netai and Guha, Subhasis K and Goswami, Rama Prosad and Saha, Shiben K and Ali, Nahid (1999) Differential Decline in Leishmania Membrane Antigen-Specific Immunoglobulin G (IgG), IgM, IgE, and IgG Subclass Antibodies in Indian Kala-Azar Patients after Chemotherapy. Infection and Immunity, 67 (12). pp. 6663-6669.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/673/