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A novel method for prognostic evaluation of childhood acute lymphoblastic leukemia

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Title A novel method for prognostic evaluation of childhood acute lymphoblastic leukemia
 
Creator Sinha, D
Mandal, Chitra
Bhattacharyya, DK
 
Subject Infectious Diseases and Immunology
 
Description Although childhood acute lymphoblastic leukemia (ALL) is
highly responsive to chemotherapy, patients in remission may
harbor residual leukemic blasts, the cause of disease persistence
and resurgence.1 Improved assays are therefore needed
to evaluate individual chemotherapeutic response and predict
impending relapse. Employing a 9-O acetyl sialic acid binding
lectin, ATNH, we have identified two 9-O acetylated sialoglycoconjugates
(9-OAcSGs), as novel biomarkers on leukemic
blasts of newly diagnosed/untreated ALL patients2 of both B
and/or T lineages and assessed their differential expression at
different phases of therapy.3 Presently, we describe a noninvasive,
blood-based lymphoproliferation assay to evaluate the clinical status of B lineage ALL patients (HLA-DR+,CD19+, n
= 16) followed up longitudinally for 3 years.
ALL patients, who received treatment as per the UKALLVIII
protocol, were bled at different phases of therapy. Peripheral
blood mononuclear cells were isolated and cultured with
ATNH (0.05–12 mg) for 96 h followed by an 18 h pulse with
3H-TdR. Radioactivity incorporated, in individual samples,
was plotted against ATNH dose and the dose corresponding to
maximal incorporation of radioactivity was designated as its
‘maximal lymphoproliferative dose’ (MLD). The MLD was
employed to evaluate the percentage recovery of individual
patients.
 
Publisher Nature Publishing Group
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/709/1/LEUKEMIA_13(_2)_309%2D312;1999[80].pdf
Sinha, D and Mandal, Chitra and Bhattacharyya, DK (1999) A novel method for prognostic evaluation of childhood acute lymphoblastic leukemia. Leukemia, 13 (2). pp. 309-312.
 
Relation htp://dx.doi.org/10.1038/sj.leu.2401312
http://www.eprints.iicb.res.in/709/