A mannose-binding glycoprotein found in the 4 day post coital rat uterus is involved in pregnancy
EPrints@IICB
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Title |
A mannose-binding glycoprotein found in the 4 day post coital rat uterus is involved in pregnancy |
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Creator |
Mehta, Monika
Chowdhury, Mridula |
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Subject |
Cell Biology & Physiology
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Description |
Experiments were conducted to study the functional implication of a mannose-binding glycoprotein found in the day 4 post coital (p.c.) rat uterus, using a mono-specific polyclonal antibody raised against the glycoprotein. Western Blot and immunohistochemical techniques were employed to study the distribution of the glycoprotein, and the results suggest that this glycoprotein is present only in the day 4 p.c. uterus and is specifically localized in the stromal cells. Administration of anti-UA (Uterine Agglutinin) antiserum against the glycoprotein into the day 4 p.c. uterine lumen inhibits carrying of embryo to term. The antiserum is not embryo toxic. After in vivo in utero intra-luminal administration of anti-UA antiserum in day 4 p.c. rat the antiserum has been specifically localized in the uterine stroma by immunohistochemistry. After intravenous injection, the glycoprotein is cleared mainly through the kidney and liver. The possible role of this glycoprotein in the implantation process in rats has been discussed. From the data it is evident that UA may not be directly involved in sugar-sugar interactions with embryo since it is not present in any significant amount in pregnant uterus from day 5 onwards. Since other experiments show that UA does have some role to play in early pregnancy, UA probably acts through some other factor, and preliminary studies suggest that this factor maybe TGF-b3. (Mol Cell Biochem 195: 65–75, 1999) |
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Publisher |
Kluwer
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Date |
1999
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/714/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY_195_(1%2D2)_65%2D75;1999[59].pdf
Mehta, Monika and Chowdhury, Mridula (1999) A mannose-binding glycoprotein found in the 4 day post coital rat uterus is involved in pregnancy. Molecular and Cellular Biochemistry, 195 (1-2). pp. 65-75. |
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Relation |
http://dx.doi.org/10.1023/A:1006901206170
http://www.eprints.iicb.res.in/714/ |
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