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Import of RNA into Leishmania Mitochondria Occurs through Direct Interaction with Membrane-bound Receptors

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Title Import of RNA into Leishmania Mitochondria Occurs through
Direct Interaction with Membrane-bound Receptors
 
Creator Mahapatra, Sridam
Adhya, Samit
 
Subject Molecular & Human Genetics
 
Description Cytoplasmic tRNAs are imported into the kinetoplast
mitochondrion of Leishmania, but the mechanism of import
is unknown, particularly whether RNA is transferred
as a ribonucleoprotein complex through the protein
import pathway or by a distinct receptor-mediated
mechanism. Using isolated mitochondria, it was shown
that a small, importable RNA, which is structurally homologous to tRNA, binds rapidly, specifically, and with
high affinity to the mitochondrial surface in the absence
of soluble protein factors to form an import intermediate.
Two classes of binding site of apparent Kd 0.3 and 10
nM, respectively, were distinguished. tRNA from Leishmania,
but not yeast, competitively inhibited the binding.
Northwestern blot analysis revealed the presence of
a 15-kDa RNA binding protein on the mitochondrial surface.
Whereas receptor binding was resistant to heparin
and KCl, internalization was sensitive to both reagents.
These results are consistent with the presence of a direct
mechanism of receptor-mediated RNA import on
Leishmania mitochondria.
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/715/1/JOURNAL_OF_BIOLOGICAL_CHEMISTRY%2C_271(_34)%2C_20432%2D20437[24].pdf
Mahapatra, Sridam and Adhya, Samit (1996) Import of RNA into Leishmania Mitochondria Occurs through Direct Interaction with Membrane-bound Receptors. The Journal of Biological Science, 271 (34). pp. 20432-20437.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/715/