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Chemokine-Induced Leishmanicidal Activity in Murine Macrophages via the Generation of Nitric Oxide

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Title Chemokine-Induced Leishmanicidal Activity in Murine Macrophages
via the Generation of Nitric Oxide
 
Creator Bhattacharyya, Sandip
Ghosh, Sanjukta
Dasgupta, Biplab
Mazumder, Debashis
Roy, Syamal
Majumdar, Subrata
 
Subject Infectious Diseases and Immunology
 
Description This study explored the role of the proinflammatory chemokines macrophage inflammatory
protein (MIP)–1a and macrophage chemoattractant protein (MCP)–1 for development of antileishmanial
activity. There was substantial inhibition in nitrite generation in Leishmania donovani–
infected macrophages. A marked elevation of nitrite generation and induction of inducible
nitric oxide (NO) synthase (iNOS) mRNA was found in chemokine-primed parasite-infected
macrophages. Tumor necrosis factor–a, which is the priming signal for NO production, was
also up-regulated under similar experimental conditions. The priming with chemokine inhibited
the multiplication of L. donovani amastigotes within the intramacrophageal milieu. The antileishmanial
effect of chemokines was almost completely abrogated when the macrophages were
preincubated with L-N-monomethyl arginine, the specific inhibitor of iNOS. The results of this
investigation suggest that the CC chemokinesMIP-1a andMCP-1 orchestrate an antileishmanial
armamentarium via the induction of an NO-mediated regulatory mechanism to control the intracellular
growth and multiplication of the Leishmania protozoan.
 
Publisher University of Chicago Press
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/741/1/JOURNAL_OF_INFECTIOUS_DISEASES_185(12)1704%2D1708;2002[47].pdf
Bhattacharyya, Sandip and Ghosh, Sanjukta and Dasgupta, Biplab and Mazumder, Debashis and Roy, Syamal and Majumdar, Subrata (2002) Chemokine-Induced Leishmanicidal Activity in Murine Macrophages via the Generation of Nitric Oxide. The Journal of Infectious Diseases, 185 (12). pp. 1704-1708.
 
Relation http://dx.doi.org/10.1086/340820
http://www.eprints.iicb.res.in/741/