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Resistance to Cadmium and Zinc in Acidiphilium symbioticum KM2 Is Plasmid Mediated

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Title Resistance to Cadmium and Zinc in Acidiphilium symbioticum KM2 Is
Plasmid Mediated
 
Creator Mahapatra, Nitish R
Ghosh, Sajalendu
Deb, Chirajyoti
Banerjee, Pataki C
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description The acidophilic heterotroph, Acidiphilium symbioticum KM2, is highly resistant to several
metals and harbors three plasmids of 3.8, 7.1, and 56 kb in size. The bacterium becomes extremely
sensitive to metals when it is cured of its plasmids. A mini-plasmid library was constructed by ligating
the plasmid DNA fragments generated by MboI partial digestion into the BamHI site of pBluescriptII
KS�. The Lac�Ampr transformants of Escherichia coli DH5�, isolated after transformation with the
library, were counter-selected on Cu2�, Cd2�, Ni2�, and Zn2�-containing plates. Only Cd2�- and
Zn2�-resistant colonies were developed, and, after screening, four types of recombinant plasmids
designated as pNM201 (7.2 kb), pNM206 (3.4 kb), pNM208 (4.5 kb), and pNM215 (4.9 kb) were
obtained. The DNA insert in pNM206 hybridized strongly with the 3.8-kb plasmid and weakly with the
7.1-kb plasmid of Acidiphilium symbioticum KM2. The DNA insert in pNM215 hybridized only with the
7.1-kb plasmid. These results strongly suggested that resistance to cadmium and zinc in A. symbioticum
KM2 is mediated by these plasmids. The smallest insert of 422 bp in pNM206 conferring metal resistance
in E. coli has no sequence similarity with the reported metal-resistant genes. All the putative ORFs are
significantly rich (up to 37%) in basic amino acids, mainly arginine.
 
Publisher Springer Verlag
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/751/1/CURRENT_MICROBIOLOGY_45_(1)_70%2D73;2002[44].pdf
Mahapatra, Nitish R and Ghosh, Sajalendu and Deb, Chirajyoti and Banerjee, Pataki C (2002) Resistance to Cadmium and Zinc in Acidiphilium symbioticum KM2 Is Plasmid Mediated. Current Microbiology, 45 (3). pp. 180-186.
 
Relation http://dx.doi.org/10.1007/s00284-001-0113-6
http://www.eprints.iicb.res.in/751/