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Infectivity and Virulence of Leishmania donovani Promastigotes: a Role for Media, Source, and Strain of Parasite

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Title Infectivity and Virulence of Leishmania donovani Promastigotes: a Role for
Media, Source, and Strain of Parasite
 
Creator Dey, Tuhina
Afrin, Farhat
Anam, Khairul
Ali, Nahid
 
Subject Infectious Diseases and Immunology
 
Description Transformation of promastigotes of Leishmania donovani strain AG83 from amastigotes derived from an infected animal
was studied in three media, Schneider's Drosophila medium (SDM), Medium 199 (M199), and biphasic M199 (B-M199) with 10%
fetal bovine serum. The media, SDM and B-M199, both supported a more efficient transformation of promastigotes in comparison with
M199. Infectivity studies in hamsters and BALB/c mice showed that promastigotes isolated in B-M199 were several folds more infective
than those obtained from M199. Comparison of the infectivity and virulence of promastigotes of AG83, with a recent isolate of kalaazar,
SL94, harvested under similar conditions, revealed greater infectivity of SL94 for both macrophages and animal models. The
present study demonstrates that the medium used for the conversion of amastigotes to promastigotes plays a major role in determining
the infectivity of the freshly transformed L. donovani promastigotes in hamsters and BALB/c mice. The source and the strain of the
parasite also influence the outcome of L. donovani infection
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/765/1/JOURNAL_OF_EUKARYOTIC_MICROBIOLOGY_49(_4)270%2D274;2002[43].pdf
Dey, Tuhina and Afrin, Farhat and Anam, Khairul and Ali, Nahid (2002) Infectivity and Virulence of Leishmania donovani Promastigotes: a Role for Media, Source, and Strain of Parasite. Journal of Eukaryotic Microbiology, 49 (4). pp. 270-274.
 
Relation http://dx.doi.org/10.1111/j.1550-7408.2002.tb00369.x
http://www.eprints.iicb.res.in/765/