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The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium

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Title The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium
 
Creator Mohanakumar, K P
Samanta Ray, S
Biisselberg, D
 
Subject Cell Biology & Physiology
 
Description Effects of the neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its metabolite, 1-methyl-4-phenyl pyridinium (MPP+) were investigated on neuronal membrane currents and intrasynaptosomal calcium levels employing whole cell patch clamp and FURA-2AM respectively. Membrane currents elicited by voltage ramps as well as voltage activated potassium-currents were studied in the cultured rat dorsal root ganglia (DRG) neurons. These neurons contained 0.36 ± 0.04 pmol/mg of dopamine as detected employing HPLC-electrochemistry. MPTP and MPP+ (1 μM) respectively caused significant increase (25 ± 4%) or inhibition (53 ± 10%) of the total membrane current. Potassium-currents elicited by voltage jumps from –80 to 0 mV were significantly inhibited (68 ± 8%) by MPP+, but MPTP caused no effect. MPP+-induced increase in the FURA-2 fluorescence signal was insensitive to extrasynaptosomal calcium, indicated intrasynaptosomal mobilization by the neurotoxin. These results suggest involvement of ion channels in MPTP neurotoxicity, and imply its role in the etiopathology of Parkinson’s disease
 
Publisher John Wiley & Sons
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/777/1/NEUROSCIENCE_RESEARCH_COMMUNICATIONS__30(_1_)_35%2D42;2002[82].pdf
Mohanakumar, K P and Samanta Ray, S and Biisselberg, D (2002) The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium. Neuroscience Research Communications, 30 (1). pp. 35-42. ISSN 0893-6609
 
Relation http://dx.doi.org/10.1002/nrc.10015
http://www.eprints.iicb.res.in/777/