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Sialic acid binding protein of human endometrium: Its regulation by steroids

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Title Sialic acid binding protein of human endometrium:
Its regulation by steroids
 
Creator Sen, Shanta
Chowdhury, Geeta
Chowdhury, Mridula
 
Subject Chemistry
 
Description In the present study, we have observed that sialic acid binding protein (SABP – a 54 kDa glycoprotein which was isolated from
human endometrial scrapings taken at various stages of the menstrual cycle from normal cycling females and purified to apparent
homogeneity and was earlier reported from this laboratory) was found in sufficiently detectable amount in the endometrium
of normal cycling women whereas it was found in lesser amount in tissue from women who have recently entered the postmenopause
stage. SABP was observed in both follicular and luteal phase of menstrual cycle which was found by western blot
analysis. In the de-novo synthesis experiment, synthesis and secretion of SABP was found to be stimulated by estradiol (E2)
whereas progesterone (P4) was found to have no significant stimulatory effect on it which was also confirmed by HEC cell
culture. In the HEC cell culture, priming of cells with E2 was found to influence the effect of P4 on SABP when it was added 2 h after E2 administration. This was observed by doing immunoprecipitation followed by SDS-PAGE and autoradiography.
Hence this report clearly indicates that E2 regulates the synthesis and secretion of 54 kDa SABP from human endometrium. How E2 priming of endometrium influences the effect of P4 on SABP has been discussed.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/876/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY%2C_221(_1%2D2)%2C_17%2D23%2C2001[42].pdf
Sen, Shanta and Chowdhury, Geeta and Chowdhury, Mridula (2001) Sialic acid binding protein of human endometrium: Its regulation by steroids. Molecular and Cellular Biochemistry, 221 (1-2). pp. 17-23.
 
Relation http://dx.doi.org/10.1023/A:1010901303113
http://www.eprints.iicb.res.in/876/