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Cholera Toxin (CTX) Genetic Element in Vibrio cholerae 0139

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Title Cholera Toxin (CTX) Genetic Element in
Vibrio cholerae 0139
 
Creator Bhadra, Rupak K
Roychoudhury, Susanta
Banerjee, Rajat K
Kar, Sujata
Majumdar, Ruma
Sengupta, Sanghamitra
Chatterjee, Soma
Khetawat, GopaI
Das, Jyotirmoy
 
Subject Infectious Diseases and Immunology
Molecular & Human Genetics
 
Description PFGE analysis of the Notl- and Sfil-digested genome of Virbrio cholerae 0139
strains isolated from different epidemic regions of India showed that all the
strains are of clonal origin and the genome size is about 2.2 Mb. An analysis of
the electrophoretic profiles of the genome of 0139 strains, the RFLP of the
cholera toxin (age)ne and Southern blot hybridization of Notidigested
genomes of classical, El Tor and 0139 with a Noti-linking clone of classical
strain 5698, suggest that these strains closely resemble V. cholerae 01 biotype
El Tor, but are widely different from the classical 01 vibrios. Using restriction
enzymes which cleave a single site in either the core region or in the direct
repeat sequence (RS) of the CTX genetic element, it has been shown that the
genome of most of the 0139 strains has two copies of the ctx gene in tandem
connected by two RSs. The chromosomal location of the CTX genetic element
in the 0139 strain is the same as that reported for El Tor vibrios. The
organization of the virulence gene cassettes in different 0139 strains shows
genetic heterogeneity in the population. Whilst most of the epidemic strains
have two copies of the CTX genetic element, in some strains the number of
elements has been amplified and in at least one strain a single copy of the
element has been deleted.
 
Date 1995
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/913/1/MICROBIOLOGY%2DUK__141%2C__1977%2D1983;1995[30].pdf
Bhadra, Rupak K and Roychoudhury, Susanta and Banerjee, Rajat K and Kar, Sujata and Majumdar, Ruma and Sengupta, Sanghamitra and Chatterjee, Soma and Khetawat, GopaI and Das, Jyotirmoy (1995) Cholera Toxin (CTX) Genetic Element in Vibrio cholerae 0139. Microbiology, 141. pp. 1977-1983.
 
Relation http://dx.doi.org/10.1099/13500872-141-8-1977
http://www.eprints.iicb.res.in/913/