Complete Soluble Antigen from a UDP-Galactose:N-Acetylglucosamine β 1-4 Galactosyltransferase expressing Leishmania donovani promastigote induces complete protection in an experimental model of visceral leishmaniasis’
EPrints@IICB
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Title |
Complete Soluble Antigen from a UDP-Galactose:N-Acetylglucosamine β 1-4 Galactosyltransferase expressing Leishmania donovani promastigote induces complete protection in an experimental model of visceral leishmaniasis’
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Creator |
Bhaumik , Siddhartha Kumar
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Subject |
Infectious Diseases and Immunology
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Description |
This disquisition is an attempt to formulate an effective immunoprophylactic and immunotherapeutic agent against protozoan Leishmania donovani, the causative pathogen for fatal visceral leishmaniasis (VL), commonly known as Kala-azar in India. Vaccination against this genetically diverse protozoan should be the most preferred approach for combating this disease, especially since chemotherapy is expensive, difficult to administer and is often ineffective due to the emerging drug resistance strains. Leishmania vaccine development has proven to be a difficult and challenging task, which is mostly hampered by inadequate knowledge of parasite pathogenesis and the complexity of immune responses needed for protection. At present most of the vaccines currently in market against tuberculosis, BCG, polio, measles and mumps are in the form of live attenuated vaccines. Though, several vaccination strategies against experimental leishmaniasis have been attempted, a successful vaccine against the disease has been elusive. Moreover, compared to cutaneous leishmaniasis, vaccination against visceral leishmaniasis has received limited attention. Our constant endeavor in search of an antileishmanial vaccine lead us to identify an UDP-Galactose:N-acetylglucosamine β 1–4 galactosyltransferase (GalT) expressing attenuated Leishmania parasite which can be used as live vaccine against visceral leishmaniasis. The live, attenuated vaccines are known to stimulate helper T-cell response and antibody immunity. The major problem of attenuation reversal could not be detected in these genetically defined stable clonal leishmanial parasites under long period of in vitro culture, making them risk-free. |
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Date |
2009
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Type |
Thesis
NonPeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/921/1/Thesis_of_Siddhartha_Kumar_Bhaumik.pdf
Bhaumik , Siddhartha Kumar (2009) Complete Soluble Antigen from a UDP-Galactose:N-Acetylglucosamine β 1-4 Galactosyltransferase expressing Leishmania donovani promastigote induces complete protection in an experimental model of visceral leishmaniasis’. PhD thesis, Jadavpur University. |
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Relation |
http://www.eprints.iicb.res.in/921/
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