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Role of Superoxide Dismutase in Survival of Leishmania Within the Macrophage

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Title Role of Superoxide Dismutase in Survival of Leishmania Within the Macrophage
 
Creator Ghosh, Sanjay
Goswami, Srikanta
Adhya, Samit
 
Subject Molecular & Human Genetics
 
Description Intracellular parasitic protozoans of the genus Leishmania depend
for their survival on the elaboration of enzymic and other
mechanisms for evading toxic free-radical damage inØicted by
their phagocytic macrophage host. One such mechanism may
involve superoxide dismutase (SOD), which detoxiÆes reactive
superoxide radicals produced by activated macrophages, but the
role of this enzyme in parasite survival has not yet been
demonstrated. We have cloned a SOD gene from L. tropica and
generated SOD-deÆcient parasites by expressing the correspond- ing antisense RNA from an episomal vector. Such parasites have enhanced sensitivity to menadione and hydrogen peroxide in axenic culture, and a markedly reduced survival in mouse macrophages. These results indicate that SOD is a major determinant of intracellular survival of Leishmania.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/936/1/BIOCHEMICAL_JOURNAL%2C369%2C_447%2D452[82].pdf
Ghosh, Sanjay and Goswami, Srikanta and Adhya, Samit (2003) Role of Superoxide Dismutase in Survival of Leishmania Within the Macrophage. Biochemical Journal, 369. pp. 447-452.
 
Relation http://dx.doi.org/10.1042/BJ20021684
http://www.eprints.iicb.res.in/936/