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Sugar Receptor Mediated Drug delivery to Macrophages in the Therapy of Experimental Visceral Leishmaniasis

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Title Sugar Receptor Mediated Drug delivery to Macrophages in the
Therapy of Experimental Visceral Leishmaniasis
 
Creator Chakraborty, Prasanta
Bhaduri, Amar Nath
Das, Pijush K
 
Subject Infectious Diseases and Immunology
 
Description Methotrexate (MTX) conjugate of a neoglycoprotein, mannosyl
bovine serum albumin, containing an average of 30 moles of MTX per mole
of neoglycoprotein was taken up efficiently by murine peritoneal macrop
hages through cell surface mannosy 1 receptors. The conjugate strongly
inhibits the growth of Leishmania donovani inside macrophages. with 50%
inhibitory dose of 0.11 pglml MTX, which makes it 100 times more active
than free MTX (50% inhibitory dose of 12.1 yg/ml). MTX conjugated to
BSA or other non-specific neoglycoproteins like galactose-BSA and glucose-
BSA have leishmanicidal effects comparable to free MTX. Moreover, in
a murine model of experimental visceral leishmaniasis, the drug conjugate
reduced the spleen parasite burden by more than 85% in a 30 day model
whereas the same concentration of free drug caused little effect. The results
demonstrate that neoglycoproteins may be useful as carriers for receptor
mediated drug delivery to treat macrophage associated diseases.
 
Publisher Elsevier
 
Date 1990
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/951/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__166(_1_)_404%2D410;199%2D[54].pdf
Chakraborty, Prasanta and Bhaduri, Amar Nath and Das, Pijush K (1990) Sugar Receptor Mediated Drug delivery to Macrophages in the Therapy of Experimental Visceral Leishmaniasis. Biochemical and Biophysical Research Communications, 166 (1). pp. 404-410.
 
Relation http://dx.doi.org/10.1016/0006-291X(90)91959-V
http://www.eprints.iicb.res.in/951/