Record Details

Mechanism of Erythrocyte Death in Human Population Exposed to Arsenic Through Drinking Water

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Mechanism of Erythrocyte Death in Human Population Exposed to Arsenic Through Drinking Water
 
Creator Biswas, Debabrata
Banerjee, Mayukh
Sen, Gargi
Das, Jayanta K
Banerjee, Apurba
Sau, Tanmoy Jyoti
Pandit, Sudipta
Giri, Ashok K
Biswas, Tuli
 
Subject Cell Biology & Physiology
 
Description Arsenic contamination in drinking water is one of the biggest natural calamities, which has become an imperative threat to human health throughout
the world.Abbreviation of erythrocyte lifespan leading to the development of anemia is a common sequel in arsenic exposed population. This study was
undertaken to explore the mechanism of cell death in human erythrocytes during chronic arsenic exposure. Results revealed transformation of smooth
discoid red cells into evaginated echinocytic form in the exposed individuals. Further distortion converted reversible echinocytes to irreversible
spheroechinocytes. Arsenic toxicity increased membrane microviscosity along with an elevation of cholesterol/phospholipid ratio, which hampered the
flexibility of red cell membrane andmade themless deformable. Significant increase in the binding ofmerocyanine 540 with erythrocyte membrane due
to arsenic exposure indicated disruption of lipid packing in the outer leaflet of the cell membrane resulting from altered transbilayer phospholipid
asymmetry. Arsenic induced eryptosis was characterized by cell shrinkage and exposure of phosphatidylserine at the cell surface. Furthermore,
metabolic starvation with depletion of cellular ATP triggered apoptotic removal of erythrocytes from circulation. Significant decrease in reduced
glutathione content indicating defective antioxidant capacity was coupled with enhancement of malondialdehyde and protein carbonyl levels, which
pointed to oxidative damage to erythrocyte membrane. Arsenic toxicity intervened into red cell membrane integrity eventually leading to membrane
destabilization and hemoglobin release. The study depicted the involvement of both erythrophagocytosis and hemolysis in the destruction of human
erythrocytes during chronic arsenic exposure.
 
Publisher Elsevier
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1028/1/TAAP_%2D%2D_2008.pdf
Biswas, Debabrata and Banerjee, Mayukh and Sen, Gargi and Das, Jayanta K and Banerjee, Apurba and Sau, Tanmoy Jyoti and Pandit, Sudipta and Giri, Ashok K and Biswas, Tuli (2008) Mechanism of Erythrocyte Death in Human Population Exposed to Arsenic Through Drinking Water. Toxicology and Applied Pharmacology, 230. pp. 57-66.
 
Relation http://dx.doi.org/10.1016/j.taap.2008.02.003
http://www.eprints.iicb.res.in/1028/