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Role of Protein-tyrosine Phosphatases on b-adrenergic Receptor Mediated Morphological Differentiation of Astrocytes

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Title Role of Protein-tyrosine Phosphatases on b-adrenergic Receptor Mediated Morphological Differentiation of Astrocytes
 
Creator Gharami, Kusumika
Das, Sumantra
 
Subject Cell Biology & Physiology
 
Description A role of protein-tyrosine phosphatases in isoproterenol induced differentiation of cultured astrocytes was investigated. Unlike
serine/threonine phosphatase inhibitors, the tyrosine phosphatase inhibitor, sodium orthovanadate effectively blocked transformation
of the polygonal astrocytes to process bearing stellate cells on exposure to isoproterenol for 2 days. Isoproterenol caused a
stimulation of c-AMP dependent protein kinase activity in the cells only at the initial stages (45 min) and at 12 and 24 h, there was a
decline in the level of phospho-tyrosinated proteins which could be antagonised by the protein kinase A inhibitor, H89. Genestein, a
protein-tyrosine kinase inhibitor, had no effect on the alteration in the morphology of the astroglial cells induced by isoproterenol
but by itself, decreased the dephosphorylation of the phospho-tyrosinated proteins, the decline being less than that observed in
isoproterenol treated cells. Moreover, unlike H89, genestein had no effect on isoproterenol-induced dephosphorylation of phosphotyrosinated
proteins. Taken together it appears that the dephosphorylation of tyrosine residues during isoproterenol-induced
astrocyte differentiation is a downstream event of protein kinase A stimulation and needs to attain a critical level in order for the
cells to differentiate.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1035/1/2003%2DJCNA.pdf
Gharami, Kusumika and Das, Sumantra (2003) Role of Protein-tyrosine Phosphatases on b-adrenergic Receptor Mediated Morphological Differentiation of Astrocytes. Journal of Chemical Neuroanatomy, 26. pp. 125-132.
 
Relation http://dx.doi.org/10.1016/S0891-0618(03)00045-0
http://www.eprints.iicb.res.in/1035/