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Protein Tyrosine Phosphorylation of a Heparin-Binding Sperm Membrane M(HBSM) is Associated with Capacitation and Acrosome Reaction

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Title Protein Tyrosine Phosphorylation of a Heparin-Binding Sperm
Membrane M(HBSM) is Associated with Capacitation
and Acrosome Reaction
 
Creator Mor, Visesato
Das, Tapasi
Bhattacharjee, Maitreyi
Chatterjee, Tapati
 
Subject Cell Biology & Physiology
 
Description Protein tyrosine phosphorylation in spermatozoa is associated with epididymal maturation and though to be central for attainment of
a capacitated state and expression of hyperactivated motility. Heparin, the most highly sulfated glycosaminoglycans, was also the most
potent at stimulating the acrosomal reaction in bovine epididymal spermatozoa. Studies using radiolabeled inorganic phosphate showed
11-fold increase 32Pi incorporation in heparin-binding sperm membrane protein (HBSM) during spermatozoal capacitation, and the
phosphorylation occurs at the tyrosine residue. Epididymal spermatozoa were induced to undergo capacitation and acrosome reaction
by 70% when the cells were incubated in BWW medium supplemented with heparin. The spermatozoa pre-treated with anti-HBSM antibody
showed 46% reduction in the hyperactivated motility and lowers the acrosome reaction. This was confirms by measuring the hydrolysis
of benzoyl-L-arginine ethyl ether (BAEE) by the acrosomal enzyme; acrosin. The preliminary finding suggests that HBSM may play
an important role in the sperm capacitation and acrosome reaction
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1046/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS_352_(2)404%2D409;2007[124].pdf
Mor, Visesato and Das, Tapasi and Bhattacharjee, Maitreyi and Chatterjee, Tapati (2007) Protein Tyrosine Phosphorylation of a Heparin-Binding Sperm Membrane M(HBSM) is Associated with Capacitation and Acrosome Reaction. Biochemical and Biophysical Research Communications, 352 (2). pp. 404-409.
 
Relation http://dx.doi.org/10.1016/j.bbrc.2006.11.026
http://www.eprints.iicb.res.in/1046/