Record Details

Fatty acid Represses Insulin Receptor Gene Expression by Impairing HMGA1 through Protein Kinase Ce

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Fatty acid Represses Insulin Receptor Gene Expression by Impairing HMGA1 through Protein Kinase Ce
 
Creator Dey, Debleena
Bhattacharya, Anirban
Roy, Sib Sankar
Bhattacharya, Samir
 
Subject Cell Biology & Physiology
 
Description It is known that free fatty acid (FFA) contributes to the development of insulin resistance and type2 diabetes. However, the underlying
mechanism in FFA-induced insulin resistance is still unclear. In the present investigation we have demonstrated that palmitate significantly
(p < 0.001) inhibited insulin-stimulated phosphorylation of PDK1, the key insulin signaling molecule. Consequently, PDK1
phosphorylation of plasma membrane bound PKCe was also inhibited. Surprisingly, phosphorylation of cytosolic PKCe was greatly
stimulated by palmitate; this was then translocated to the nuclear region and associated with the inhibition of insulin receptor (IR) gene
transcription. A PKCe translocation inhibitor peptide, eV1, suppressed this inhibitory effect of palmitate, suggesting requirement of
phospho-PKCe migration to implement palmitate effect. Experimental evidences indicate that phospho-PKCe adversely affected
HMGA1. Since HMGA1 regulates IR promoter activity, expression of IR gene was impaired causing reduction of IR on cell surface
and that compromises with insulin sensitivity.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1047/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__357(_2)_474%2D479;2007[79].pdf
Dey, Debleena and Bhattacharya, Anirban and Roy, Sib Sankar and Bhattacharya, Samir (2007) Fatty acid Represses Insulin Receptor Gene Expression by Impairing HMGA1 through Protein Kinase Ce. Biochemical and Biophysical Research Communications, 357 (2). pp. 474-479.
 
Relation http://dx.doi.org/10.1016/j.bbrc.2007.03.183
http://www.eprints.iicb.res.in/1047/