Record Details

Extracellular Trehalose Utilization by Saccharomyces Cerevisiae

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Extracellular Trehalose Utilization by Saccharomyces Cerevisiae
 
Creator Basu, Arghya
Bhattacharyya, Soma
Chaudhuri , Paramita
Sengupta, Subhabrata
Ghosh, Anil K
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Two haploid strains of Saccharomyces cerevisiae viz. MATĪ± and MATa were grown in glucose and trehalose medium and growth patterns were
compared. Both strains show similar growth, except for an extended lag phase in trehalose grown cells. In both trehalose grown strains increase in
activities of both extracellular trehalase activities and simultaneous decrease in extracellular trehalose level was seen. This coincided with a sharp increase
in extracellular glucose level and beginning of log phase of growth. Alcohol production was also observed. Secreted trehalase activity was detected, in
addition to periplasmic activity. It appeared that extracellular trehalose was hydrolyzed into glucose by extracellular trehalase activity. This glucose was
utilized by the cells for growth. The alcohol formation was due to the fermentation of glucose. Addition of extracellular trehalase caused reduction in the
lag phase when grown in trehalose medium, supporting our hypothesis of extracellular utilization of trehalose.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1106/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA%2DGENERAL_SUBJECTS__Volume_1760___Issue_2___Pages_134%2D140_[132].pdf
Basu, Arghya and Bhattacharyya, Soma and Chaudhuri , Paramita and Sengupta, Subhabrata and Ghosh, Anil K (2006) Extracellular Trehalose Utilization by Saccharomyces Cerevisiae. Biochimica et Biophysica Acta, 1760 (2). pp. 134-140.
 
Relation http://dx.doi.org/10.1016/j.bbagen.2005.11.016
http://www.eprints.iicb.res.in/1106/