Record Details

Purification and Characterization of a Low Molecular Weight Multifunctional Cytotoxic Phospholipase A2 from Russell’s Viper Venom.

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Purification and Characterization of a Low Molecular Weight Multifunctional Cytotoxic Phospholipase A2 from Russell’s Viper Venom.
 
Creator Maity, Gargi
Mandal, Somnath
Chatterjee, Amitabha
Bhattacharyya, Debasish
 
Subject Structural Biology & Bioinformatics
 
Description A basic toxin from Russell’s viper venom of 7.2 kDa (RVV-7) has been purified to homogeneity after partial unfolding by 4M urea followed by
filtration through Centricon-30 membrane. Its N-terminal sequence showed strong homology with snake venom cytotoxins. Cytotoxic activity of
RVV-7 has been demonstrated with B16F10 melanoma cells. PLA2 activity was observed in cytotoxin (CX3) from Naja kauthia bearing sequence
homology with RVV-7. Phospholipase A2 and trypsin inhibitory activities were also observed with RVV-7. Chemical modification and inhibition
studies suggested independent functional sites for these activities. A qualitative assessment of tumor growth inhibition by RVV-7 has been made.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1138/1/JOURNAL_OF_CHROMATOGRAPHY_B%2DANALYTICAL_TECHNOLOGIES_IN_THE_BIOMEDICAL_AND_LIFE_SCIENCES__845(2)_Pages_232%2D243;_2007[123].pdf
Maity, Gargi and Mandal, Somnath and Chatterjee, Amitabha and Bhattacharyya, Debasish (2007) Purification and Characterization of a Low Molecular Weight Multifunctional Cytotoxic Phospholipase A2 from Russell’s Viper Venom. Journal of Chromatography B, 845. pp. 232-243.
 
Relation http://dx.doi.org/10.1016/j.jchromb.2006.08.038
http://www.eprints.iicb.res.in/1138/