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Indian Black Scorpion (Heterometrus bengalensis Koch) Venom induced Antiproliferative and Apoptogenic Activity against Human Leukemic Cell Lines U937 and K562.

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Title Indian Black Scorpion (Heterometrus bengalensis Koch)
Venom induced Antiproliferative and Apoptogenic Activity against Human Leukemic Cell Lines U937 and K562.
 
Creator Gupta, Shubho Das
Debnath, Anindita
Saha, Archita
Giri, Biplab
Tripathi, Gayatri
Vedasiromoni , J R
Gomes, Antony
Gomes, Aparna
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Venoms are rich source of several bioactive compounds that possess therapeutic potentials. The different constituents of scorpion venom
can modulate cell proliferation, cell growth and cell cycle. In the present communication, the cytotoxic activity of Indian black scorpion
(Heterometrus bengalensis) venom was explored on human leukemic U937 and K562 cells. Scorpion venom induced U937 and K562 cell
growth inhibition and the IC50 value calculated to be 41.5 �g/ml (U937) and 88.3 �g/ml (K562). The scorpion venom showed characteristic
features of apoptosis such as membrane blebbing, chromatin condensation and DNA degradation in both the cells as evidenced by confocal,
fluorescence, scanning electron microscopy. Scorpion venom (IC50 dose, 48 h) induced DNA fragmentation as evidenced by comet formation.
Flow-cytometric assay revealed a significant amount of apoptotic cells (early and late) due to scorpion venom treatment. The venom induced
cell cycle arrest was observed with maximum cell accumulation at sub-G1 phase. Thus, the Indian scorpion (H. bengalensis) venom possessed
antiproliferative, cytotoxic and apoptogenic activity against human leukemic cells.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1146/1/LEUKEMIA_RESEARCH_31_(6)_817%2D825;2007[80].pdf
Gupta, Shubho Das and Debnath, Anindita and Saha, Archita and Giri, Biplab and Tripathi, Gayatri and Vedasiromoni , J R and Gomes, Antony and Gomes, Aparna (2007) Indian Black Scorpion (Heterometrus bengalensis Koch) Venom induced Antiproliferative and Apoptogenic Activity against Human Leukemic Cell Lines U937 and K562. Leukemia Research, 31 (6). pp. 817-825.
 
Relation http://dx.doi.org/10.1016/j.leukres.2006.06.004
http://www.eprints.iicb.res.in/1146/