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Non-Coding pDNA Bearing Immunostimulatory Sequences co-Entrapped with Leishmanial Antigens in Cationic Liposomes Elicits almost Complete Protection Against Experimental Visceral Leishmaniasis in BALB/c Mice.

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Title Non-Coding pDNA Bearing Immunostimulatory Sequences
co-Entrapped with Leishmanial Antigens in Cationic Liposomes Elicits almost Complete Protection Against Experimental Visceral Leishmaniasis in BALB/c Mice.
 
Creator Mazumder, Saumyabrata
Ravindran, Rajesh Ravindran
Banerjee, Antara Banerjee
Ali, Nahid
 
Subject Infectious Diseases and Immunology
 
Description The difficulty in making successful vaccines against leishmaniasis is partly due to lack of an appropriate adjuvant. Non-coding plasmidDNA
(pDNA) bearing immunostimulatory sequences (ISS) is a potent activator of innate immunity, and can thus act as an adjuvant with vaccine
antigen. We therefore evaluated the efficacy of pDNA and soluble leishmanial antigens (SLA) to protect against challenge with Leishmania
donovani infection. We demonstrate that immunomodulatory activity of pDNA, which potentiated a Th1 immune responses, led to enhanced
protection with SLA. Importantly, adding cationic liposomes as vehicle to the antigen, with pDNA either complexed or entrapped within,
significantly increased the potentiating effect of pDNA. Further, comparison of the two vaccine formulations demonstrated an impressive
increase in the protective efficacy up to two folds when both antigen and pDNA were within the vehicle. Thus, these studies establish the
utility of non-coding pDNA bearing ISS as strong promoters of vaccine potency of liposomal antigens especially when co-entrapped with the
antigen in cationic liposomes.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1160/1/VACCINE__25_(_52)__8771%2D8781;2007[4].pdf
Mazumder, Saumyabrata and Ravindran, Rajesh Ravindran and Banerjee, Antara Banerjee and Ali, Nahid (2007) Non-Coding pDNA Bearing Immunostimulatory Sequences co-Entrapped with Leishmanial Antigens in Cationic Liposomes Elicits almost Complete Protection Against Experimental Visceral Leishmaniasis in BALB/c Mice. Vaccine, 25 (52). pp. 8771-8781.
 
Relation http://dx.doi.org/10.1016/j.vaccine.2007.10.028
http://www.eprints.iicb.res.in/1160/