Nonsteroidal Anti-Inflammatory Drugs Inhibit Gastric Peroxidase Activity.
EPrints@IICB
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Title |
Nonsteroidal Anti-Inflammatory Drugs Inhibit Gastric Peroxidase Activity. |
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Creator |
Banerjee, Ranajit K
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Subject |
Cell Biology & Physiology
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Description |
The peroxidase activity of the mitochondrial fraction of rat gastric mucosa was inhibited with various nonsteroidal anti-inflammatory drugs (NSAIDs) in vitro. Indomethacin was found to be more effective than phenylbutazone (PB) or acetylsalicylic acid (ASA). Mouse gastric peroxidase was also very sensitive to indomethacin inhibition. Indomethacin has no significant effect on submaxillary gland peroxidase activity of either of the species studied. Purified rat gastric peroxidase activity was inhibited 75% with 0.15 mM indomethacin showing half-maximal inhibition at 0.04 mM. The inhibition could be withdrawn by increasing the concentration of iodide but not by H202. NSAIDs inhibit gastric peroxidase activity more effectively at acid pH (pH 5.2) than at neutral pH. Spectral studies showed a bathochromic shift of the Soret band of the enzyme with indomethacin indicating its interaction at or near the heme part of the enzyme. |
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Publisher |
Elsevier
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Date |
1990
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/1216/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA__1034(_3_)275%2D2801990[32].pdf
Banerjee, Ranajit K (1990) Nonsteroidal Anti-Inflammatory Drugs Inhibit Gastric Peroxidase Activity. BBA - Biochimica et Biophysica Acta, 1034 (3). pp. 275-280. |
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Relation |
http://dx.doi.org/10.1016/0304-4165(90)90050-7
http://www.eprints.iicb.res.in/1216/ |
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