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Interactions of Berberine with Poly(A) and tRNA

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Title Interactions of Berberine with Poly(A) and tRNA
 
Creator Nandi, R
Debnath, D
Maiti, Motilal
 
Subject Chemistry
 
Description The interaction of berberine chloride with poly(A) and tRNA has been studied by various spectroscopic techniques.
Binding parameters determined from spectrophotometric and spectrofluorimetric measurements by Scatchard analysis
indicate a very high effective binding capacity of berberine to poly(A) as compared to DNA or tRNA. The circular
dichroism studies show that binding of berberine to poly(A) causes a significant change in the circular dichroic spectrum
of poly(A) itself, as manifested by (i) a decrease of both positive and negative bands and (ii) appearance of a
conservative type of extrinsic circular dichroic spectrum in the wavelength range of 300-400 nm, while it does not cause
any significant alteration to the A form structure of tRNA. It is concluded that berberine interacts stronger with poly
(A) than DNA or tRNA. The results are interpreted in terms of its reported biological activities.
 
Publisher Elsevier
 
Date 1990
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1218/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA__1049__(3_)339%2D342;1990[26].pdf
Nandi, R and Debnath, D and Maiti, Motilal (1990) Interactions of Berberine with Poly(A) and tRNA. BBA - Biochimica et Biophysica Acta, 1049 (3). pp. 339-342.
 
Relation http://dx.doi.org/10.1016/0167-4781(90)90107-D
http://www.eprints.iicb.res.in/1218/