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Binding of Estradiol-Peroxidase Conjugate to Estrogen Receptor

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Field Value
 
Title Binding of Estradiol-Peroxidase Conjugate to
Estrogen Receptor
 
Creator Bhattacharjee, Maitreyi
Ali, Esahak
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description The binding of estradiol-horseradish peroxidase conjugate to rat uterine cytosolic
estrogen receptor was studied. The conjugate having a steroid to enzyme ratio of 2.8 : 1 was
allowed to bind to protamine precipitated receptor in presence or absence of 100-fold excess
of free estradiol. The bound enzyme activity was measured and the data subjected to Scatchard
analysis to obtain the dissociation constant and the number of binding sites. Although the
binding parameter so obtained differed from values obtained using radiolabelled estradiol, the
method may be used for comparative studies.
 
Publisher Elsevier
 
Date 1990
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1224/1/JOURNAL_OF_STEROID_BIOCHEMISTRY_AND_MOLECULAR_BIOLOGY__37(_5)_693%2D696;1990[1].pdf
Bhattacharjee, Maitreyi and Ali, Esahak (1990) Binding of Estradiol-Peroxidase Conjugate to Estrogen Receptor. The Journal of Steroid Biochemistry and Molecular Biology, 37 (5). pp. 693-696.
 
Relation http://dx.doi.org/10.1016/0960-0760(90)90353-M
http://www.eprints.iicb.res.in/1224/