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Increased Enzyme Secretion by 2-Deoxy-D-Glucose in Presence of Succinate by Suppression of Metabolic Enzymes in Termitomyces Clypeatus.

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Title Increased Enzyme Secretion by 2-Deoxy-D-Glucose in Presence of Succinate by Suppression of Metabolic Enzymes in Termitomyces Clypeatus.
 
Creator Pal, Swagata
Banik, Samudra Prosad
Ghorai, Shakuntala
Chowdhury, Sudeshna
Khowala, Suman
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Regulatory mode of secretion of proteins was detected for the industrial glycosidase, cellobiase, under
secreting conditions (in presence of TCA cycle intermediates like succinate etc.) in the filamentous fungus
Termitomyces clypeatus. The titers of key metabolic enzymes were investigated under secreting and nonsecreting
conditions of growth and compared to the corresponding production of intra and extracellular
levels of cellobiase. Results were compared in presence of 2-deoxy-D-glucose, a potent glycosylation
inhibitor in the secreting media. Inclusion of 2-deoxy-D-glucose in presence of succinate caused about
10 to 100 times decrease in titers of the metabolic enzymes hexokinase, fructose-1,6-bisphosphatase, isocitrate
lyase and malate dehydrogenase leading to increased secretion of cellobiase by more than 100
times. The intracellular concentration of cAMP (86-fold decrease in presence of 2-deoxy-D-glucose under
secreting conditions) and turnover rate of proteins also dropped significantly. In this suppressed metabolic
state, a 10-fold increase in the titer of the secreted cellobiase was noticed. The results indicated elucidation
of carbon catabolite repression like phenomenon in the fungus under secreting conditions which
was more pronounced by 2-deoxy-D-glucose. The interdependence between secretion and regulation of
metabolic enzymes will help in better understanding of the physiology of these highly adapted organisms
for increasing their secretion potential of glycosidases like cellobiase with high industrial value.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1243/1/CARBOHYDRATE_RESEARCH__346_(15)_2426%2D2431;2011[12].pdf
Pal, Swagata and Banik, Samudra Prosad and Ghorai, Shakuntala and Chowdhury, Sudeshna and Khowala, Suman (2011) Increased Enzyme Secretion by 2-Deoxy-D-Glucose in Presence of Succinate by Suppression of Metabolic Enzymes in Termitomyces Clypeatus. Carbohydrate Research, 346 (15). pp. 2426-2431.
 
Relation http://dx.doi.org/10.1016/j.carres.2011.08.012
http://www.eprints.iicb.res.in/1243/