Record Details

Potent Spermicidal Effect of Oleanolic acid 3-Beta-D-gGlucuronide, an Active Principle Isolated from the Plant Sesbania Sesban Merrill.

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Potent Spermicidal Effect of Oleanolic acid 3-Beta-D-gGlucuronide, an Active Principle Isolated from the Plant Sesbania Sesban Merrill.
 
Creator Das, Nilanjana
Chandran, Poornima
Chakraborty, Smritinath
 
Subject Cell Biology & Physiology
 
Description The spermicidal activity of oleanolic acid 3-β-D-glucuronide (OAG), an active principle isolated from root extracts of
Sesbania sesban, was evaluated.
Under the Sander–Cramer test criteria, the sperm-immobilizing activity of OAG was studied using highly motile rat sperm.
Sperm mortality and membrane integrity were assessed by supravital staining, hypo-osmotic swelling (HOS), transmission electron
microscopy (TEM) and sperm membrane lipid peroxidation (LPO). In vitro microbicidal potential and hemolytic index of OAG were
examined in Lactobacillus culture and rat red blood corpuscles (RBCs), respectively. Post-intravaginal OAG application, the in vivo
contraceptive efficacy was evaluated in rats. Ames test determined the carcinogenic potential of OAG.
The minimum effective concentration (MEC) of OAG was 50 mcg/mL. More than 97% of the OAG-treated sperm lost their HOS
responsiveness in a dose-dependent manner. TEM and LPO revealed that OAG affected the sperm membrane integrity. OAG declined
fertility to zero, was nonmutagenic and was not harmful to lactobacillus.
OAG has significant spermicidal activity that may be explored further.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1246/1/CONTRACEPTION___83(_2)_167%2D175;2011[130].pdf
Das, Nilanjana and Chandran, Poornima and Chakraborty, Smritinath (2011) Potent Spermicidal Effect of Oleanolic acid 3-Beta-D-gGlucuronide, an Active Principle Isolated from the Plant Sesbania Sesban Merrill. Contraception, 83 (2). pp. 167-175.
 
Relation http://dx.doi.org/10.1016/j.contraception.2010.05.009
http://www.eprints.iicb.res.in/1246/