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Association of Cholera Toxin with Vibrio Cholerae outer Membrane Vesicles which are Internalized by Human Intestinal Epithelial Cells

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Title Association of Cholera Toxin with Vibrio Cholerae outer Membrane Vesicles which
are Internalized by Human Intestinal Epithelial Cells
 
Creator Chatterjee, Debashree
Chaudhuri, Keya
 
Subject Molecular & Human Genetics
 
Description Cholera toxin (CT) is the major virulence factor of pathogenic Vibrio cholerae. The present study
demonstrates that a fraction of CT is associated with the outer membrane vesicles (OMVs) released
by V. cholerae. Atomic force microscopy (AFM) and also transmission electron microscopy (TEM) of
purified OMVs from toxigenic V. cholerae O395 revealed spherical shaped vesicles of size range 20–
200 nm. Immunoblotting of purified OMVs with polyclonal anti-CT antibody and GM1-ganglioside
dependent ELISA suggest that CT is associated with OMVs. CHO cell assay indicated that OMV associated
CT is physiologically active. OMVs labeled with fluorescent dye interacted with intestinal epithelial
cells via the CT-receptor and were internalized increasing the cAMP level. Thus OMVs may
represent an important vehicle in delivering CT to epithelial cells.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1259/1/FEBS_LETTERS__585(_9)_1357%2D1362;2011;[89].pdf
Chatterjee, Debashree and Chaudhuri, Keya (2011) Association of Cholera Toxin with Vibrio Cholerae outer Membrane Vesicles which are Internalized by Human Intestinal Epithelial Cells. FEBS Letters, 585 (9). pp. 1357-1362.
 
Relation http://dx.doi.org/10.1016/j.febslet.2011.04.017
http://www.eprints.iicb.res.in/1259/