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Cytochrome P450 Inhibitory Potential of Triphala—A Rasayana from Ayurveda

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Title Cytochrome P450 Inhibitory Potential of Triphala—A Rasayana from Ayurveda
 
Creator Ponnusankar, S
Pandita, Subrata
Babub, Ramesh
Bandyopadhyay, Arun
Mukherjee, Pulok K
 
Subject Cell Biology & Physiology
 
Description Ethnopharmacological relevance: ‘Triphala’ is one of the age-old, most commonly used polyherbal preparation
from Ayurveda as Rasayana drug.
Aim of the study: This study was aimed at evaluating the effect of ‘Triphala’ on drug modulating enzymes
to assess its safety through its potential to interact with co-administered drugs.
Materials and methods: The cytochrome P450 inhibitory effect of ‘triphala’ formulation was investigated
on rat liver microsomes using CYP450–CO complex assay and on individual isoform such as CYP3A4
and 2D6 using fluorescence screening. RP-HPLC method was developed to standardize ‘triphala’ and its
individual components using gallic acid as analytical marker compound.
Results: RP-HPLC analysis demonstrated the presence of gallic acid (4.30±2.09 mg/g) in the formulation.
The formulation showed 23% inhibition of the rat liver microsomes through CYP450–CO complex assay
which is comparatively less when compared with the individual components. Further, the effect of standardized
formulation dissolved in ethanol showed CYP3A4 and CYP2D6 inhibitory activity at the IC50
values of 119.65±1.91�g/ml and 105.03±0.98�g/ml respectively. Gallic acid was also found to inhibit
both the isoforms at the IC50 values of 87.24±1.11�g/ml and 92.03±0.38�g/ml respectively.
Conclusions: Various concentrations of the formulation and its individual components showed significantly
less inhibitory activity (p < 0.001) on individual isoforms when compared with the positive control.
Assessment on the in vitro effect of ‘triphala’ on drug modulating enzymes has important implications
for predicting the likelihood of herb–drug interactions if these are administered concomitantly.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1288/1/JOURNAL_OF_ETHNOPHARMACOLOGY__133__(1)120%2D125;2011[135].pdf
Ponnusankar, S and Pandita, Subrata and Babub, Ramesh and Bandyopadhyay, Arun and Mukherjee, Pulok K (2011) Cytochrome P450 Inhibitory Potential of Triphala—A Rasayana from Ayurveda. Journal of Ethnopharmacology, 133 (1). pp. 120-125.
 
Relation http://dx.doi.org/10.1016/j.jep.2010.09.022
http://www.eprints.iicb.res.in/1288/