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Spectroscopic Characterization of the Interaction of Phenosafranin and Safranin O with Double Stranded, Heat Denatured and Single Stranded Calf Thymus DNA

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Title Spectroscopic Characterization of the Interaction
of Phenosafranin and Safranin O with Double Stranded,
Heat Denatured and Single Stranded Calf Thymus DNA
 
Creator Saha, Ishita
Kumar, G Suresh
 
Subject Chemistry
 
Description Interaction of phenosafranin and safranin O with
double stranded, heat denatured and single stranded calf
thymus DNA has been studied by fluorescence, absorbance
and circular dichroic techniques. Binding to the double
stranded and heat denatured DNA conformations induced
strong quenching in the fluorescence spectra of both dyes.
Linear Scatchard plots indicated the binding to be of one
type and the affinity evaluated to be of the order of 105 M−1
with double stranded and heat denatured DNAs. Fluorescence
quenching was much weaker with the single stranded
DNA and the binding affinity was one order lower.
Ferrocyanide quenching studies revealed that the fluorescence
emission of the dye molecules bound to the double
stranded and heat denatured DNAs was quenched much
less compared to that bound to the single stranded DNA.
Further, there was significant emission polarization for the
bound dyes and strong energy transfer from the DNA base
pairs to the dye molecules indicating intercalative binding.
Salt dependence of the binding phenomenon revealed that
electrostatic forces have significant role in the binding process.
The intercalation of these molecules to double stranded and
heat denatured DNA and simple stacking to single strands was
proved by these fluorescence techniques. Support to the
fluorescence results have been derived from absorption and
circular dichroic results. Phenosafranin was revealed to be a
stronger binding species compared to safranin
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1290/1/JOURNAL_OF_FLUORESCENCE___21(_1)_247%2D255;2011[146].pdf
Saha, Ishita and Kumar, G Suresh (2011) Spectroscopic Characterization of the Interaction of Phenosafranin and Safranin O with Double Stranded, Heat Denatured and Single Stranded Calf Thymus DNA. Journal of Fluorescence, 21 (1). pp. 247-255.
 
Relation http://dx.doi.org/10.1007/s10895-010-0712-3
http://www.eprints.iicb.res.in/1290/