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UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay

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Title UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial
Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay
 
Creator Banerjee, Nupur
Bhattacharyya, Debasish
 
Subject Structural Biology & Bioinformatics
 
Description UDP-glucose dehydrogenase from Capra hircus has been purified to homogeneity by salt fractionations,
heat treatment and chromatographic steps. It is a homohexamer of about 300 kDa. Though the basic physical
and enzymatic properties of the caprine enzyme are comparable to those of the beef liver enzyme, it
has lower energy of activation and different entropy and enthalpy for the transition state during catalysis.
The caprine enzyme can act suitably as an auxiliary enzyme in the coupled assay system for UDP-galactose
4-epimerase
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1296/1/JOURNAL_OF_MOLECULAR_CATALYSIS_B%2DENZYMATIC__68(_1)_37%2D43_;2011[155].pdf
Banerjee, Nupur and Bhattacharyya, Debasish (2011) UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay. Journal of Molecular Catalysis B: Enzymatic, 68 (1). pp. 37-43.
 
Relation http://dx.doi.org/10.1016/j.molcatb.2010.09.007
http://www.eprints.iicb.res.in/1296/