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Interaction of Gold Nanoparticle with Human Serum Albumin (HSA) Protein Using Surface Energy Transfer.

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Title Interaction of Gold Nanoparticle with Human Serum Albumin (HSA) Protein Using Surface Energy Transfer.
 
Creator Sen, Tapasi
Mandal, Sadananda
Haldar, Shubhasis
Chattopadhyay, Krishnananda
Patra, Amitava
 
Subject Structural Biology & Bioinformatics
 
Description Here, we study the human serum albumin (HSA) protein�Au
nanoparticle interaction to identify the specific binding site of protein with
nanoparticles by using the surface energy transfer (SET) method among
tryptophan (Trp) of HSA, ANS-dye-labeled HSA protein, and Au nanoparticles.
Here, ANS dye is used as a probe located at domain IIIA of HSA. In particular,
absorbance, fluorescence quenching, decay time, circular dichroism, dynamic
light scattering, and TEM measurements are performed to understand the
physical properties of protein-conjugated Au nanoparticles. Using the SET
method, the measured distances between the Trp residue of HSA and the
binding site of HSA interacting with Au nanoparticles are 42.5, 41.9, and 48.1 Å
for 1.5, 2.0, and 2.9 nm HSA-conjugated Au nanoparticles, respectively. The
measured distances between the binding site of ANS dye (located at domain
IIIA) in HSA to the binding site of HSA interacting with Au nanoparticles are 51,
51.5, and 54.7 Å for 1.5, 2.0, and 2.9 nm HSA-conjugated Au nanoparticles, respectively. From the protein structural data (using
PyMol software), the distances from the center of domain IIIA to Cys53�Cys62 disulfide bond and Trp to Cys53�Cys62 disulfide
bond are obtained to be 51.5 and 39.1 Å, respectively. Thus, the distances calculated by using SET equation (Trp to Au binding site
distance and ANS to Au binding site distance) nicely match with the distances obtained from protein structural data by using PyMol
software. Analysis suggests that the Au nanoparticle is attached to HSA by linkage through Cys53�Cys62 disulfide bond which is
located at subdomain IA of HSA.
 
Publisher American Chemical Society
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1300/1/JOURNAL_OF_PHYSICAL_CHEMISTRY_C___115_(49)_24037%2D24044;2011[2].pdf
Sen, Tapasi and Mandal, Sadananda and Haldar, Shubhasis and Chattopadhyay, Krishnananda and Patra, Amitava (2011) Interaction of Gold Nanoparticle with Human Serum Albumin (HSA) Protein Using Surface Energy Transfer. The Journal of Physical Chemistry C, 115 (49). pp. 24037-24044.
 
Relation http://dx.doi.org//10.1021/jp207374g
http://www.eprints.iicb.res.in/1300/