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Changes in the Leaf Proteome Profile of Mentha Arvensis in response to Alternaria Alternata Infection

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Title Changes in the Leaf Proteome Profile of Mentha Arvensis in
response to Alternaria Alternata Infection
 
Creator Sinha, Ragini
Chattopadhyay, Sharmila
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Plant pathogenic fungi cause important yield losses in crops. A proteomic approach was
used to study the changes in the leaf proteome profile of the plant Mentha arvensis infected
with a necrotrophic fungus, Alternaria alternata. High-resolution two-dimensional gel
electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric
analysis was used to identify highly abundant proteins differentially expressed in response
to fungal infection. From a total of 210 reproducibly detected and analyzed spots, the
intensity of sixty-seven spots was altered, and forty-five of them were successfully
identified by matrix assisted laser desorption/ionization time of flight-mass spectrometry
(MALDI TOF/TOF MS/MS). Fifty-six percent of the identified proteins belonged to energy and
metabolism whereas 29% were stress and defense related. Taken together, the results allow
to assess changes at the proteomic level in the host due to the defense response. Results
show an initial defense response, not strong enough to overcome the pathogenesis, which
may be similar to other susceptible plant–pathogen interactions; however, cross-talks
between various defense pathways, regulatory networks and physiological conditions are
other important aspects to be considered.
 
Publisher American Chemical Society
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1303/1/JOURNAL_OF_PROTEOMICS__74(_3)_327%2D336;2011[126].pdf
Sinha, Ragini and Chattopadhyay, Sharmila (2011) Changes in the Leaf Proteome Profile of Mentha Arvensis in response to Alternaria Alternata Infection. Journal of Proteome Research, 74 (3). pp. 327-336.
 
Relation http://dx.doi.org/10.1016/j.jprot.2010.11.009
http://www.eprints.iicb.res.in/1303/