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Interference of Sugars in the Coomassie Blue G dye Binding Assay of Proteins

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Title Interference of Sugars in the Coomassie Blue G dye Binding Assay of Proteins
 
Creator Banik, Samudra Prosad
Pal, Swagata
Ghorai, Shakuntala
Chowdhury, Sudeshna
Khowala, Suman
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description The presence of sugars causes significant deviation from the actual absorbance of proteins in the Bradford
protein assay. In these studies, polysaccharides and disaccharides at milligram levels mimicked proteins
in microgram equivalents. Monosaccharides, which individually did not show any absorbance, interfered
significantly by sequestering the dye species. The studies demonstrated that in a mixture of sugars and
proteins, sugar interference was much higher than expected from sugar molecules’ individual contribution.
Estimated protein values were increased 2 to 4 times after precipitation from fungal culture broths.
Thus, in carbohydrate-rich samples, protein concentrations should be ascertained by precipitation from
crude extracts and resolubilization in a noninterfering buffer
 
Publisher Elsevier
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1354/1/ANALYTICAL_BIOCHEMISTRY___386(_1_)_113%2D115;2009[94].pdf
Banik, Samudra Prosad and Pal, Swagata and Ghorai, Shakuntala and Chowdhury, Sudeshna and Khowala, Suman (2009) Interference of Sugars in the Coomassie Blue G dye Binding Assay of Proteins. Analytical Biochemistry, 386 (1). pp. 113-115.
 
Relation http://dx.doi.org/10.1016/j.ab.2008.12.006
http://www.eprints.iicb.res.in/1354/