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Studies on Substrate Specificity and Activity Regulating Factors of Trehalose-6-Phosphate Synthase of Saccharomyces Cerevisiae

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Title Studies on Substrate Specificity and Activity Regulating Factors of
Trehalose-6-Phosphate Synthase of Saccharomyces Cerevisiae
 
Creator Chaudhuri, Paramita
Basu, Arghya
Sengupta, Shinjinee
Lahiri, Sagar
Dutta, Trina
Ghosh, Anil K
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Purified trehalose-6-phosphate synthase (TPS) of Saccharomyces cerevisiae was effective over a wide range of
substrates, although differing with regard to their relative activity. Polyanions heparin and chondroitin
sulfate were seen to stimulate TPS activity, particularly when a pyrimidine glucose nucleotide like UDPG was
used, rather than a purine glucose nucleotide like GDPG. A high Vmax and a low Km value of UDPG show its
greater affinity with TPS than GDPG or TDPG. Among the glucosyl acceptors TPS showed maximum activity
with G-6-P which was followed by M-6-P and F-6-P. Effect of heparin was also extended to the purification of
TPS activity, as it helped to retain both stability and activity of the final purified enzyme. Metal co-factors,
specifically MnCl2 and ZnCl2 acted as stimulators, while enzyme inhibitors had very little effect on TPS
activity. Metal chelators like CDTA, EGTA stimulated enzyme activity by chelation of metal inhibitors.
Temperature and pH optima of the purified enzyme were determined to be 40 °C and pH 8.5 respectively.
Enzyme activity was stable at 0–40 °C and at alkaline pH.
 
Publisher Elsevier
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1355/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA%2DGENERAL_SUBJECTS___1790_(_5)_368%2D374;2009[76].pdf
Chaudhuri, Paramita and Basu, Arghya and Sengupta, Shinjinee and Lahiri, Sagar and Dutta, Trina and Ghosh, Anil K (2009) Studies on Substrate Specificity and Activity Regulating Factors of Trehalose-6-Phosphate Synthase of Saccharomyces Cerevisiae. BBA - Biochimica et Biophysica Acta, 1790 (5). pp. 368-374.
 
Relation http://dx.doi.org/10.1016/j.bbagen.2009.03.008
http://www.eprints.iicb.res.in/1355/