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Nanoencapsulation of Quercetin Enhances its Dietary Efficacy in Combating Arsenic-Induced Oxidative Damage in Liver and Brain of Rats

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Title Nanoencapsulation of Quercetin Enhances its Dietary Efficacy in Combating Arsenic-Induced Oxidative Damage in Liver and Brain of Rats
 
Creator Ghosh, Aparajita
Mandal, Ardhendu K
Sarkar, Sibani
Panda, Subhamay
Das, Nirmalendu
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Aims: This study was performed to evaluate the therapeutic efficacy of nanocapsulated flavonoidal quercetin
(QC) in combating arsenic-induced reactive oxygen species (ROS)-mediated oxidative damage in hepatocytes
and brain cells in a rat model.
Main methods: Hepatic and neuronal cell damage in rats was made by a single injection (sc) of sodium
arsenite (NaAsO2, 13 mg/kg b. wt. in 0.5 ml of physiological saline). A single dose of 500 μl of quercetin
suspension (QC) (QC 8.98 μmol/kg) or 500 μl of nanocapsulated QC (NPQC) (QC 8.98 μmol/kg) was given
orally to rats at 90 min prior to the arsenite injection.
Key findings: Inorganic arsenic depositions (182±15.6 and 110±12.8 ng/g protein) were found in hepatic and
neuronal mitochondrial membranes. Antioxidant levels in hepatic and neuronal cells were reduced
significantly by arsenic. NPQC prevented the arsenite-induced reduction in antioxidant levels in the liver and
brain. Arsenic induced a substantial decrease in liver and brain cell membrane microviscosities, and NPQC
treatment resulted in a unique protection against the loss. A significant correlation between mitochondrial
arsenic and its conjugated diene level was observed both in liver and brain cells for all experimental rats.
Significance: Arsenic-specific antidotes are used against arsenic-induced toxicity. However, the target site is
poorly recognized and therefore achieving an active concentration of drug molecules can be a challenge.
Thus, our objective was to formulate NPQC and to investigate its therapeutic potential in an oral route against
arsenite-induced hepatic and neuronal cell damage in a rat model
 
Publisher Elsevier
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1371/1/LIFE_SCIENCES___84_(_3%2D4)_75%2D80;2009[117].pdf
Ghosh, Aparajita and Mandal, Ardhendu K and Sarkar, Sibani and Panda, Subhamay and Das, Nirmalendu (2009) Nanoencapsulation of Quercetin Enhances its Dietary Efficacy in Combating Arsenic-Induced Oxidative Damage in Liver and Brain of Rats. Life Sciences, 84 (3-4). pp. 75-80.
 
Relation http://dx.doi.org/10.1016/j.lfs.2008.11.001
http://www.eprints.iicb.res.in/1371/