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Involvement of Intracellular Signaling Cascades in Inflammatory Responses in Human Intestinal Epithelial Cells following Vibrio Cholerae Infection

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Title Involvement of Intracellular Signaling Cascades in Inflammatory Responses in Human Intestinal Epithelial Cells following Vibrio Cholerae Infection
 
Creator Bandyopadhaya, Arunava
Das, Debalina
Chaudhuri, Keya
 
Subject Molecular & Human Genetics
 
Description Vibrio cholerae, the etiological agent of cholera, leads to the induction of host cell nuclear responses and the
activation of proinflammatory cytokines in the cultured intestinal epithelial cells. However, the host cell
signaling pathway leading to proinflammatory response is not explored. In this study, we demonstrated
that V. cholerae infection on intestinal epithelial cells results in the activation of extracellular signalregulated
kinases1/2(ERK1/2) and p38 of the mitogen activated protein kinase (MAPK) family. V. cholerae
induced intracellular pathways in Int407 cells leading to the activation of protein kinase A (PKA) and
protein tyrosin kinase (PTK) in upstream of MAPK and nuclear factor-kappaB (NF-�B) pathway. Inhibitor
study of Ca2+ and phospholipase-gamma (PLC-�) pathway suggested the possible involvement of Ca2+
signaling in the V. cholerae pathogenesis. V. cholerae culture supernatants as also insertional mutants
of ctxA, toxR and toxT genes modulate the activation of MAPK and NF-�B signaling pathways. MAPK
and NF-�B signaling pathway activation were also modulated by adherence and motility of V. cholerae.
Studies with inhibitor of NF-�B, MAPK, PTK, PKA, PKC, Ca2+ and PLC pathways showed differential cytokine
secretion in Int407 following V. cholerae infection. Therefore V. cholerae mediated induction of nuclear
responses through signal transduction pathway and subsequent activation of proinflammatory cytokines
in Int407 modulated by V. cholerae secretory factors, virulence, adhesion/motility which might explain
some of its reactogenic mechanisms
 
Publisher Elsevier
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1375/1/MOLECULAR_IMMUNOLOGY__46(_6_)_1129%2D1139_;2009[91].pdf
Bandyopadhaya, Arunava and Das, Debalina and Chaudhuri, Keya (2009) Involvement of Intracellular Signaling Cascades in Inflammatory Responses in Human Intestinal Epithelial Cells following Vibrio Cholerae Infection. Molecular Immunology, 46 (6). pp. 1129-1139.
 
Relation http://dx.doi.org/10.1016/j.molimm.2008.11.003
http://www.eprints.iicb.res.in/1375/