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Stringent Response in Vibrio Cholerae: Genetic Analysis of spoT Gene Function and Identification of a Novel (p)ppGpp Synthetase Gene

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Title Stringent Response in Vibrio Cholerae: Genetic Analysis of
spoT Gene Function and Identification of a Novel (p)ppGpp
Synthetase Gene
 
Creator Das, Bhabatosh
Pal, Ritesh Ranjan
Bag, Satyabrata
Bhadra, Rupak K
 
Subject Infectious Diseases and Immunology
 
Description RelA and SpoT of Gram-negative organisms critically
regulate cellular levels of (p)ppGpp. Here, we have
dissected the spoT gene function of the cholera
pathogen Vibrio cholerae by extensive genetic
analysis. Unlike Escherichia coli, V. cholerae DrelA
DspoT cells accumulated (p)ppGpp upon fatty acid
or glucose starvation. The result strongly suggests
RelA-SpoT-independent (p)ppGpp synthesis in
V. cholerae. By repeated subculturing of a V. cholerae
DrelA DspoT mutant, a suppressor strain with
(p)ppGpp0 phenotype was isolated. Bioinformatics
analysis of V. cholerae whole genome sequence
allowed identification of a hypothetical gene
(VC1224), which codes for a small protein (~29 kDa)
with a (p)ppGpp synthetase domain and the gene is
highly conserved in vibrios; hence it has been named
relV. Using E. coli DrelA or DrelA DspoT mutant we
showed that relV indeed codes for a novel (p)ppGpp
synthetase. Further analysis indicated that relV gene
of the suppressor strain carries a point mutation at
nucleotide position 676 of its coding region (DrelA
DspoT relV676), which seems to be responsible for
the (p)ppGpp0 phenotype. Analysis of a V. cholerae
DrelA DspoT DrelV triple mutant confirmed that apart
from canonical relA and spoT genes, relV is a
novel gene in V. cholerae responsible for (p)ppGpp
synthesis.
 
Publisher Blackwell Publishing
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1376/1/MOLECULAR_MICROBIOLOGY___72(_2)_380%2D398;2009[82].pdf
Das, Bhabatosh and Pal, Ritesh Ranjan and Bag, Satyabrata and Bhadra, Rupak K (2009) Stringent Response in Vibrio Cholerae: Genetic Analysis of spoT Gene Function and Identification of a Novel (p)ppGpp Synthetase Gene. Molecular Microbiology, 72 (2). pp. 380-398.
 
Relation http://dx.doi.org/10.1111/j.1365-2958.2009.06653.x
http://www.eprints.iicb.res.in/1376/