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Simultaneous Enzyme Immunoassay for the Screening of Aflatoxin B1 and Ochratoxin A in Chili Samples

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Title Simultaneous Enzyme Immunoassay for the Screening of
Aflatoxin B1 and Ochratoxin A in Chili Samples
 
Creator Saha, Debjani
Acharya, Debopam
Roy, Dipika
Shrestha, Dilip
Dhar, Tarun K
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Membrane-based immunoassay has been developed for simultaneous estimation of aflatoxin B1 (AFB1) and ochratoxin A (OA) in chili samples.
The combined estimation of both the mycotoxins is more economical in respect of time, work and materials than two separate assays. The method
uses a lowcost test device consisting of a membrane with immobilized anti-AFB1 and anti-OAantibodies and a filter paper attached to a polyethylene
card below the membrane. It allows direct analysis of sample extracts containing substantial amount (40%) of methanol. This permits the use
of two-fold diluted sample extracts resulting in minimum dilution error. The limit of quantitation obtained was 2 and 10 �g kg−1 for AFB1 and
OA, respectively. The tolerance of 40% methanol was found to be due to the application of small size (0.8mm diameter) spots on membranes,
as the tolerance decreases to 20% with gradual increase in spot size. The combined method is capable of producing acceptable results to analyze
AFB1 and OA in chili with accuracy and precision. The AFB1 and OA values obtained for spiked and naturally contaminated chili samples by the
simultaneous method were in good correlation with those measured by individual ELISA. The method offers a simple, rapid and cost-effective
screening tool to meet the requirements of the rapidly evolving EU legislation.
 
Publisher Elsevier
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1396/1/ANALYTICA_CHIMICA_ACTA___584_(2)_343%2D349;2007[111].pdf
Saha, Debjani and Acharya, Debopam and Roy, Dipika and Shrestha, Dilip and Dhar, Tarun K (2007) Simultaneous Enzyme Immunoassay for the Screening of Aflatoxin B1 and Ochratoxin A in Chili Samples. Analytica Chimica Acta, 584 (2). pp. 343-349.
 
Relation http://dx.doi.org/10.1016/j.aca.2006.11.042
http://www.eprints.iicb.res.in/1396/