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Redox Manipulation: An Approach for Preferential Killing of Cancer Cells by Oxidative Stress Inducers.

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Title Redox Manipulation: An Approach for Preferential Killing of Cancer Cells by Oxidative Stress Inducers.
 
Creator Biswas, Nabendu
 
Subject Cancer Biology and Inflammatory Disorder Division
 
Description The beginning of modern era of chemotherapy can be traced back to 1942 with the first use of nitrogen mustards drugs. Though some other modes of treatment like radiation therapy emerged successively, they didn’t bring complete cure. As a result chemotherapeutic approach have come under the
focus worldwide. Several decades of intensive study in this area revealed a number of mechanisms through which they mediate cell death by apoptosis and/or necrosis.
Reactive oxygen species (ROS) are normal metabolic byproducts that are generated continuously in cells. Redox manipulation is emerging as a therapeutic approach to treat cancer. Opposite effects of intracellular ROS on
normal cells versus cancer cells have recently been hypothesized. As the basal level of intracellular ROS is low in normal cells, its increase up to certain
extent is expected to be associated with cell proliferation. On the other hand, higher threshold of intracellular ROS in tumor cells, close to the threshold of
cytotoxicity, is associated with higher cellular proliferation. Additional increase of intracellular ROS is therefore likely to inhibit tumor cell
proliferation. Intrinsic higher threshold of ROS in cancer cells has been exploited in the present study for preferential killing of cancer cells of
different origin by some ROS inducing agents. Cell death will be assessed in vitro on a number of cancer cell lines and normal human peripheral blood mononuclear cells and in vivo in nude mice model using human tumor xenografts. Apoptosis will be evaluated by flowcytometry after staining with annexin V staining and by evaluation of
caspase activation. Redox- sensitive signal transudation pathways will also be evaluated in the current study.
 
Date 2012
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1464/1/Nabendu_pdf.pdf
Biswas, Nabendu (2012) Redox Manipulation: An Approach for Preferential Killing of Cancer Cells by Oxidative Stress Inducers. PhD thesis, Jadavpur University.
 
Relation http://www.eprints.iicb.res.in/1464/