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In Vitro Initiated Sperm Forward Motility in Caput Spermatozoa: Weak and Transient

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Title In Vitro Initiated Sperm Forward Motility in Caput
Spermatozoa: Weak and Transient
 
Creator Das, K
Das, S
Bhoumik, S
Jaiswal, B S
Majumder, Gopal Chandra
Dungdung, S R
 
Subject Cell Biology & Physiology
 
Description Testicular spermatozoa during journey through epididymis acquire forward
motility, which is essential for fertility. To understand the biochemistry of
sperm motility initiation, various initiation media have been developed that
permitted high level of motility induction (55–60%) in the immature caputspermatozoa
in presence of activating principles: theophylline, bicarbonate and
epididymal plasma (EP) when analysed microscopically. Here, we show for the
first time using caprine model that stability and quality of in vitro-induced
motility in the caput spermatozoa is insignificant in contrast to naturally
induced motility in mature cauda spermatozoa. In vitro-induced motility of the
immature spermatozoa was lost completely upon the removal of these activators
by centrifugation. Selective withdrawal of either EP or HCO3 by dilution
retains 50–60% of the in vitro-induced motility. Spectrophotometric analysis
revealed that in vitro-induced vertical motility in immature spermatozoa is too
little when compared to mature spermatozoa. In in vitro-initiated caput spermatozoa,
cyclic adenosine monophosphate level becomes doubled but lesser
than cauda spermatozoa. This revelation concludes that scientific knowledge
generated over the years on the basis of in vitro initiation method is insignificant
and needs improvisation to delineate biochemical regulation of sperm
motility which in turn has remarkable potential in wide biological fields,
especially in infertility treatment.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1477/1/ANDROLOGIA__44_(_1)__807%2D812;2012[10].pdf
Das, K and Das, S and Bhoumik, S and Jaiswal, B S and Majumder, Gopal Chandra and Dungdung, S R (2012) In Vitro Initiated Sperm Forward Motility in Caput Spermatozoa: Weak and Transient. Andrologia, 44 (1). pp. 807-812.
 
Relation http://dx.doi.org/10.1111/j.1439-0272.2011.01161.x
http://www.eprints.iicb.res.in/1477/